Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-021-02742-w
Title: A high-throughput pipeline for scalable kit-free RNA extraction
Authors: Han, Ping 
Go, Maybelle K 
Chow, Jeng Yeong 
Xue, Bo 
Lim, Yan Ping 
Crone, Michael A
Storch, Marko
Freemont, Paul S 
Yew, Wen Shan 
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Issue Date: 1-Dec-2021
Publisher: NATURE PORTFOLIO
Citation: Han, Ping, Go, Maybelle K, Chow, Jeng Yeong, Xue, Bo, Lim, Yan Ping, Crone, Michael A, Storch, Marko, Freemont, Paul S, Yew, Wen Shan (2021-12-01). A high-throughput pipeline for scalable kit-free RNA extraction. SCIENTIFIC REPORTS 11 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-021-02742-w
Abstract: An overreliance on commercial, kit-based RNA extraction in the molecular diagnoses of infectious disease presents a challenge in the event of supply chain disruptions and can potentially hinder testing capacity in times of need. In this study, we adapted a well-established, robust TRIzol-based RNA extraction protocol into a high-throughput format through miniaturization and automation. The workflow was validated by RT-qPCR assay for SARS-CoV-2 detection to illustrate its scalability without interference to downstream diagnostic sensitivity and accuracy. This semi-automated, kit-free approach offers a versatile alternative to prevailing integrated solid-phase RNA extraction proprietary systems, with the added advantage of improved cost-effectiveness for high volume acquisition of quality RNA whether for use in clinical diagnoses or for diverse molecular applications.
Source Title: SCIENTIFIC REPORTS
URI: https://scholarbank.nus.edu.sg/handle/10635/218737
ISSN: 20452322
DOI: 10.1038/s41598-021-02742-w
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