Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/218217
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dc.titleINVESTIGATION OF THE EPIDERMAL GROWTH FACTOR RECEPTOR ORGANIZATION AND DYNAMICS ON THE PLASMA MEMBRANE OF LIVE CELLS USING FLUORESCENCE TECHNIQUES
dc.contributor.authorHARIKRUSHNAN BALASUBRAMANIAN
dc.date.accessioned2022-03-31T18:00:47Z
dc.date.available2022-03-31T18:00:47Z
dc.date.issued2021-07-29
dc.identifier.citationHARIKRUSHNAN BALASUBRAMANIAN (2021-07-29). INVESTIGATION OF THE EPIDERMAL GROWTH FACTOR RECEPTOR ORGANIZATION AND DYNAMICS ON THE PLASMA MEMBRANE OF LIVE CELLS USING FLUORESCENCE TECHNIQUES. ScholarBank@NUS Repository.
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/218217
dc.description.abstractThe epidermal growth factor receptor (EGFR) is a plasma membrane protein that is a crucial regulator of cell proliferation and survival, and its mutations and dysregulated signalling have been implicated in many cancers. However, a clear understanding of its oligomerization state, dynamics, and interactions with the actin cytoskeleton and other plasma membrane components has been elusive. This thesis is an investigation of these questions in live CHO-K1 cells using total internal reflection fluorescence (TIRF) microscopy-based fluorescence spectroscopy techniques. First, brightness-calibrated camera-based number and brightness (N&B) analysis was established for estimating EGFR oligomerization in resting cells, after drug treatments, and using EGFR mutants. Next, a strategy was developed to combine multiple fluorescence techniques and obtain mutually consistent structure and dynamics information of EGFR behaviour and interactions simultaneously. Cross-correlation analysis between any two parameters provided new insights into EGFR biology that could not be obtained from separate sequential measurements.
dc.language.isoen
dc.subjectEGFR, N&B, MICROSCOPY, IMAGING, FLUORESCENCE, PLASMA MEMBRANE
dc.typeThesis
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.supervisorWohland, Thorsten
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY (FOS)
dc.identifier.orcid0000-0003-4966-0221
Appears in Collections:Ph.D Theses (Restricted)

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