Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2021.113629
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dc.titleAccessible detection of SARS-CoV-2 through molecular nanostructures and automated microfluidics
dc.contributor.authorZhao, Haitao
dc.contributor.authorZhang, Yan
dc.contributor.authorChen, Yuan
dc.contributor.authorHo, Nicholas RY
dc.contributor.authorSundah, Noah R
dc.contributor.authorNatalia, Auginia
dc.contributor.authorLiu, Yu
dc.contributor.authorMiow, Qing Hao
dc.contributor.authorWang, Yu
dc.contributor.authorTambyah, Paul A
dc.contributor.authorOng, Catherine WM
dc.contributor.authorShao, Huilin
dc.date.accessioned2022-03-03T03:45:44Z
dc.date.available2022-03-03T03:45:44Z
dc.date.issued2021-12-15
dc.identifier.citationZhao, Haitao, Zhang, Yan, Chen, Yuan, Ho, Nicholas RY, Sundah, Noah R, Natalia, Auginia, Liu, Yu, Miow, Qing Hao, Wang, Yu, Tambyah, Paul A, Ong, Catherine WM, Shao, Huilin (2021-12-15). Accessible detection of SARS-CoV-2 through molecular nanostructures and automated microfluidics. BIOSENSORS & BIOELECTRONICS 194. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2021.113629
dc.identifier.issn0956-5663
dc.identifier.issn1873-4235
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/216659
dc.description.abstractAccurate and accessible nucleic acid diagnostics is critical to reducing the spread of COVID-19 and resuming socioeconomic activities. Here, we present an integrated platform for the direct detection of SARS-CoV-2 RNA targets near patients. Termed electrochemical system integrating reconfigurable enzyme-DNA nanostructures (eSIREN), the technology leverages responsive molecular nanostructures and automated microfluidics to seamlessly transduce target-induced molecular activation into an enhanced electrochemical signal. Through responsive enzyme-DNA nanostructures, the technology establishes a molecular circuitry that directly recognizes specific RNA targets and catalytically enhances signaling; only upon target hybridization, the molecular nanostructures activate to liberate strong enzymatic activity and initiate cascading reactions. Through automated microfluidics, the system coordinates and interfaces the molecular circuitry with embedded electronics; its pressure actuation and liquid-guiding structures improve not only analytical performance but also automated implementation. The developed platform establishes a detection limit of 7 copies of RNA target per μl, operates against the complex biological background of native patient samples, and is completed in <20 min at room temperature. When clinically evaluated, the technology demonstrates accurate detection in extracted RNA samples and direct swab lysates to diagnose COVID-19 patients.
dc.language.isoen
dc.publisherELSEVIER ADVANCED TECHNOLOGY
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysical Sciences
dc.subjectBiophysics
dc.subjectBiotechnology & Applied Microbiology
dc.subjectChemistry, Analytical
dc.subjectElectrochemistry
dc.subjectNanoscience & Nanotechnology
dc.subjectChemistry
dc.subjectScience & Technology - Other Topics
dc.subjectMolecular nanostructures
dc.subjectAutomated microfluidics
dc.subjectNucleic acid detection
dc.subjectElectrochemical sensor
dc.subjectCOVID-19
dc.typeArticle
dc.date.updated2022-03-03T03:34:21Z
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentDEPT OF CIVIL ENGINEERING
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentDEPT OF PHYSICS
dc.contributor.departmentEAST ASIAN INSTITUTE
dc.contributor.departmentINST FOR HEALTH INNOVATION & TECHNOLOGY
dc.contributor.departmentINSTITUTE OF SYSTEMS SCIENCE
dc.description.doi10.1016/j.bios.2021.113629
dc.description.sourcetitleBIOSENSORS & BIOELECTRONICS
dc.description.volume194
dc.published.statePublished
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