Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bios.2021.113629
DC Field | Value | |
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dc.title | Accessible detection of SARS-CoV-2 through molecular nanostructures and automated microfluidics | |
dc.contributor.author | Zhao, Haitao | |
dc.contributor.author | Zhang, Yan | |
dc.contributor.author | Chen, Yuan | |
dc.contributor.author | Ho, Nicholas RY | |
dc.contributor.author | Sundah, Noah R | |
dc.contributor.author | Natalia, Auginia | |
dc.contributor.author | Liu, Yu | |
dc.contributor.author | Miow, Qing Hao | |
dc.contributor.author | Wang, Yu | |
dc.contributor.author | Tambyah, Paul A | |
dc.contributor.author | Ong, Catherine WM | |
dc.contributor.author | Shao, Huilin | |
dc.date.accessioned | 2022-03-03T03:45:44Z | |
dc.date.available | 2022-03-03T03:45:44Z | |
dc.date.issued | 2021-12-15 | |
dc.identifier.citation | Zhao, Haitao, Zhang, Yan, Chen, Yuan, Ho, Nicholas RY, Sundah, Noah R, Natalia, Auginia, Liu, Yu, Miow, Qing Hao, Wang, Yu, Tambyah, Paul A, Ong, Catherine WM, Shao, Huilin (2021-12-15). Accessible detection of SARS-CoV-2 through molecular nanostructures and automated microfluidics. BIOSENSORS & BIOELECTRONICS 194. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2021.113629 | |
dc.identifier.issn | 0956-5663 | |
dc.identifier.issn | 1873-4235 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/216659 | |
dc.description.abstract | Accurate and accessible nucleic acid diagnostics is critical to reducing the spread of COVID-19 and resuming socioeconomic activities. Here, we present an integrated platform for the direct detection of SARS-CoV-2 RNA targets near patients. Termed electrochemical system integrating reconfigurable enzyme-DNA nanostructures (eSIREN), the technology leverages responsive molecular nanostructures and automated microfluidics to seamlessly transduce target-induced molecular activation into an enhanced electrochemical signal. Through responsive enzyme-DNA nanostructures, the technology establishes a molecular circuitry that directly recognizes specific RNA targets and catalytically enhances signaling; only upon target hybridization, the molecular nanostructures activate to liberate strong enzymatic activity and initiate cascading reactions. Through automated microfluidics, the system coordinates and interfaces the molecular circuitry with embedded electronics; its pressure actuation and liquid-guiding structures improve not only analytical performance but also automated implementation. The developed platform establishes a detection limit of 7 copies of RNA target per μl, operates against the complex biological background of native patient samples, and is completed in <20 min at room temperature. When clinically evaluated, the technology demonstrates accurate detection in extracted RNA samples and direct swab lysates to diagnose COVID-19 patients. | |
dc.language.iso | en | |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Physical Sciences | |
dc.subject | Biophysics | |
dc.subject | Biotechnology & Applied Microbiology | |
dc.subject | Chemistry, Analytical | |
dc.subject | Electrochemistry | |
dc.subject | Nanoscience & Nanotechnology | |
dc.subject | Chemistry | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | Molecular nanostructures | |
dc.subject | Automated microfluidics | |
dc.subject | Nucleic acid detection | |
dc.subject | Electrochemical sensor | |
dc.subject | COVID-19 | |
dc.type | Article | |
dc.date.updated | 2022-03-03T03:34:21Z | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.contributor.department | DEPT OF CIVIL ENGINEERING | |
dc.contributor.department | DEPT OF MEDICINE | |
dc.contributor.department | DEPT OF PHYSICS | |
dc.contributor.department | EAST ASIAN INSTITUTE | |
dc.contributor.department | INST FOR HEALTH INNOVATION & TECHNOLOGY | |
dc.contributor.department | INSTITUTE OF SYSTEMS SCIENCE | |
dc.description.doi | 10.1016/j.bios.2021.113629 | |
dc.description.sourcetitle | BIOSENSORS & BIOELECTRONICS | |
dc.description.volume | 194 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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Accessible detection of SARS-CoV-2 through molecular nanostructures and automated microfluidics.pdf | 6.31 MB | Adobe PDF | OPEN | Published | View/Download |
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