Please use this identifier to cite or link to this item: https://doi.org/10.1039/d0mh01141j
Title: Probing cell membrane damage using a molecular rotor probe with membrane-to-nucleus translocation
Authors: Wang, Kang-Nan
Qi, Guobin 
Chu, Huiying
Chao, Xi-Juan
Liu, Liu-Yi
Li, Guohui
Cao, Qian 
Mao, Zong-Wan
Liu, Bin 
Keywords: Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Materials Science, Multidisciplinary
Chemistry
Materials Science
FLUORESCENT-PROBE
PLASMA-MEMBRANE
LIPID RAFTS
VISCOSITY
CANCER
EMISSION
PENETRATION
DISEASE
DNA
Issue Date: 1-Dec-2020
Publisher: ROYAL SOC CHEMISTRY
Citation: Wang, Kang-Nan, Qi, Guobin, Chu, Huiying, Chao, Xi-Juan, Liu, Liu-Yi, Li, Guohui, Cao, Qian, Mao, Zong-Wan, Liu, Bin (2020-12-01). Probing cell membrane damage using a molecular rotor probe with membrane-to-nucleus translocation. MATERIALS HORIZONS 7 (12) : 3226-3233. ScholarBank@NUS Repository. https://doi.org/10.1039/d0mh01141j
Abstract: Damage to cell membranes, the outermost protection layer, is fatal to cells. However, precisely monitoring and in situ reporting cell membrane damage is not trivial. Herein, we present a molecular rotor probe, TPAE2, which can effectively bind to DNA and 1,2-dioleoyl-sn-glycero-3-phosphocholine in solution. Due to the light-up imaging characteristics of the molecular rotor, TPAE2 offers ultrafast and wash-free staining of plasma membrane with 160-fold fluorescence "turn-on"and excellent photostability. Once the membrane is damaged, TPAE2 can light-up the nucleus as a signal reporter. The cascade imaging of the cell membrane and nucleus using TPAE2 enabled real-Time tracking of the whole process of cell apoptosis. What's more, under irradiation, TPAE2 stained on the cell membrane could penetrate cells rapidly and selectively stain the nucleus, self-reporting the cancer cell ablation process. This is the first example that a single molecule with multiple functions can light up the nucleus as an indication of cell membrane damage. The membrane-To-nucleus translocation strategy opens up a new avenue for the design of membrane damage diagnosis probes for biomedical applications. This journal is
Source Title: MATERIALS HORIZONS
URI: https://scholarbank.nus.edu.sg/handle/10635/215105
ISSN: 2051-6347,2051-6355
DOI: 10.1039/d0mh01141j
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