Please use this identifier to cite or link to this item: https://doi.org/10.4049/jimmunol.2100844
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dc.titleCas9-Based Local Enrichment and Genomics Sequence Revision of Megabase-Sized Shark IgNAR Loci
dc.contributor.authorDong, H
dc.contributor.authorZhang, Y
dc.contributor.authorWang, J
dc.contributor.authorXiang, H
dc.contributor.authorLv, T
dc.contributor.authorWei, L
dc.contributor.authorYang, S
dc.contributor.authorLiu, X
dc.contributor.authorRen, B
dc.contributor.authorZhang, X
dc.contributor.authorLiu, L
dc.contributor.authorCao, J
dc.contributor.authorWang, M
dc.contributor.authorJIAHAI SHI
dc.contributor.authorYang, N
dc.date.accessioned2022-02-07T01:22:08Z
dc.date.available2022-02-07T01:22:08Z
dc.date.issued2022-01-01
dc.identifier.citationDong, H, Zhang, Y, Wang, J, Xiang, H, Lv, T, Wei, L, Yang, S, Liu, X, Ren, B, Zhang, X, Liu, L, Cao, J, Wang, M, JIAHAI SHI, Yang, N (2022-01-01). Cas9-Based Local Enrichment and Genomics Sequence Revision of Megabase-Sized Shark IgNAR Loci. Journal of immunology (Baltimore, Md. : 1950) 208 (1) : 181-189. ScholarBank@NUS Repository. https://doi.org/10.4049/jimmunol.2100844
dc.identifier.issn0022-1767
dc.identifier.issn1550-6606
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/214876
dc.description.abstractThe 0.8-Mb Ig new Ag receptor (IgNAR) region of the whitespotted bamboo shark (Chiloscyllium plagiosum) is incompletely assembled in Chr_44 of the reference genome. Here we used Cas9-assisted targeting of chromosome segments (CATCH) to enrich the 2 Mb region of the Chr_44 IgNAR loci and sequenced it by PacBio and next-generation sequencing. A fragment >3.13 Mb was isolated intact from the RBCs of sharks. The target was enriched 245.531-fold, and sequences had up to 94% coverage with a 255× mean depth. Compared with the previously published sequences, 20 holes were filled, with a total length of 3508 bp. In addition, we report five potential germline V alleles of IgNAR1 from six sharks that may belong to two clusters of the IgNAR. Our results provide a new method to research the germline of large Ig gene segments, as well as provide the enhanced bamboo shark IgNAR gene loci with fewer gaps.
dc.publisherThe American Association of Immunologists
dc.sourceElements
dc.subjectAnimals
dc.subjectCRISPR-Associated Protein 9
dc.subjectCRISPR-Cas Systems
dc.subjectFish Proteins
dc.subjectGenetic Loci
dc.subjectGenome
dc.subjectGenomics
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.subjectImmunoglobulins
dc.subjectReceptors, Antigen
dc.subjectSequence Analysis, DNA
dc.subjectSharks
dc.typeArticle
dc.date.updated2022-02-04T09:27:05Z
dc.contributor.departmentDEPT OF BIOCHEMISTRY
dc.description.doi10.4049/jimmunol.2100844
dc.description.sourcetitleJournal of immunology (Baltimore, Md. : 1950)
dc.description.volume208
dc.description.issue1
dc.description.page181-189
dc.published.statePublished
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