Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/214521
Title: CAPSULE BIOSYNTHESIS IN STREPTOCOCCUS PNEUMONIAE
Authors: CHEN JIA HUI
Keywords: Streptococcus pneumoniae, capsular polysaccharide, transcriptional regulation, beta-galactosidase, blue-white screening, synthetic viability
Issue Date: 1-Sep-2021
Citation: CHEN JIA HUI (2021-09-01). CAPSULE BIOSYNTHESIS IN STREPTOCOCCUS PNEUMONIAE. ScholarBank@NUS Repository.
Abstract: In Streptococcus pneumoniae, the synthesis of the capsular polysaccharide (CPS) is transcriptionally regulated to adapt to different host niches. Although many transcription factors (TFs) were implicated in controlling CPS expression, it remains unclear if all the TFs that regulate CPS expression have been discovered. Here, using a β-galactosidase (lacZ) reporter, we screened a transposon library for such TFs. Three pyruvate metabolism genes (spxB, spxR, and lctO) and one small regulatory RNA, ssrA (F32) were found to negatively and positively affect lacZ activity, respectively. However, none affected the cps expression level. This unexpected result was likely due to other factors, such as hydrogen peroxide production and sugar transport and metabolism. Besides, we developed a method to find synthetic viable (SV) pairs. SV pairs in the literature were used as a proof-of-principle to validate this method. This work highlighted the potential limitations of using lacZ reporters in S. pneumoniae.
URI: https://scholarbank.nus.edu.sg/handle/10635/214521
Appears in Collections:Master's Theses (Restricted)

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