Please use this identifier to cite or link to this item: https://doi.org/10.1523/ENEURO.0193-20.2021
Title: FEZ1 Forms Complexes with CRMP1 and DCC to Regulate Axon and Dendrite Development
Authors: Chua, Jie Yin 
Ng, Shi Jun
Yagensky, Oleksandr
Wanker, Erich E
Chua, John Jia En 
Keywords: Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
axon
CRMP1
DCC
dendrite
development
FEZ1
SNARE-MEDIATED EXOCYTOSIS
COLORECTAL-CANCER
CORTICAL-NEURONS
MEMBRANE-PROTEIN
PLASMA-MEMBRANE
TRANSPORT
GROWTH
GUIDANCE
SEMAPHORIN3A
PHOSPHORYLATION
Issue Date: 1-Mar-2021
Publisher: SOC NEUROSCIENCE
Citation: Chua, Jie Yin, Ng, Shi Jun, Yagensky, Oleksandr, Wanker, Erich E, Chua, John Jia En (2021-03-01). FEZ1 Forms Complexes with CRMP1 and DCC to Regulate Axon and Dendrite Development. ENEURO 8 (2). ScholarBank@NUS Repository. https://doi.org/10.1523/ENEURO.0193-20.2021
Abstract: Elaboration of neuronal processes is an early step in neuronal development. Guidance cues must work closely with intracellular trafficking pathways to direct expanding axons and dendrites to their target neurons during the formation of neuronal networks. However, how such coordination is achieved remains incompletely understood. Here, we char-acterize an interaction between fasciculation and elongation protein zeta 1 (FEZ1), an adapter involved in synaptic protein transport, and collapsin response mediator protein (CRMP)1, a protein that functions in growth cone guid-ance, at neuronal growth cones. We show that similar to CRMP1 loss-of-function mutants, FEZ1 deficiency in rat hippocampal neurons causes growth cone collapse and impairs axonal development. Strikingly, FEZ1-deficient neurons also exhibited a reduction in dendritic complexity stronger than that observed in CRMP1-deficient neurons, sug-gesting that the former could partake in additional developmental signaling pathways. Supporting this, FEZ1 colocalizes with VAMP2 in developing hippocampal neurons and forms a separate complex with deleted in colorectal cancer (DCC) and Syntaxin-1 (Stx1), components of the Netrin-1 signaling pathway that are also involved in regulating axon and dendrite development. Significantly, developing axons and dendrites of FEZ1-deficient neurons fail to respond to Netrin-1 or Netrin-1 and Sema3A treatment, respectively. Taken together, these findings highlight the importance of FEZ1 as a common effector to integrate guidance signaling pathways with intracellular trafficking to mediate axo-dendrite development during neuronal network formation.
Source Title: ENEURO
URI: https://scholarbank.nus.edu.sg/handle/10635/213596
ISSN: 23732822
DOI: 10.1523/ENEURO.0193-20.2021
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