Please use this identifier to cite or link to this item: https://doi.org/10.7150/thno.29101
Title: Theranostic nanodots with aggregation-induced emission characteristic for targeted and image-guided photodynamic therapy of hepatocellular carcinoma
Authors: Gao, Y.
Zheng, Q.C.
Xu, S. 
Yuan, Y. 
Cheng, X.
Jiang, S.
Kenry 
Yu, Q.
Song, Z.
Liu, B. 
Li, M.
Keywords: Aggregation-induced emission (AIE)
Hepatocellular carcinoma
Integrin ???3
Photodynamic therapy (PDT)
Theranostics
Issue Date: 2019
Publisher: Ivyspring International Publisher
Citation: Gao, Y., Zheng, Q.C., Xu, S., Yuan, Y., Cheng, X., Jiang, S., Kenry, Yu, Q., Song, Z., Liu, B., Li, M. (2019). Theranostic nanodots with aggregation-induced emission characteristic for targeted and image-guided photodynamic therapy of hepatocellular carcinoma. Theranostics 9 (5) : 1264-1279. ScholarBank@NUS Repository. https://doi.org/10.7150/thno.29101
Rights: Attribution-NonCommercial 4.0 International
Abstract: Photosensitizer (PS) serves as the central element of photodynamic therapy (PDT). The use of common nanoparticles (NPs) for PDT has typically been rendered less effective by the undesirable aggregation-caused quenching (ACQ) effect, resulting in quenched fluorescence and reduced reactive oxygen species (ROS) generation that diminish the imaging quality and PDT efficacy. To overcome the ACQ effect and to enhance the overall efficacy of PDT, herein, integrin ???3-targeted organic nanodots for image-guided PDT were designed and synthesized based on a red emissive aggregation-induced emission (AIE) PS. Methods: The TPETS nanodots were prepared by nano-precipitation method and further conjugated with thiolated cRGD (cRGD-SH) through a click reaction to yield the targeted TPETS nanodots (T-TPETS nanodots). Nanodots were characterized for encapsulation efficiency, conjugation rate, particle size, absorption and emission spectra and ROS production. The targeted fluorescence imaging and antitumor efficacy of T-TPETS nanodot were evaluated both in vitro and in vivo. The mechanism of cell apoptosis induced by T-TPETS nanodot mediated-PDT was explored. The biocompatibility and toxicity of the nanodots was examined using cytotoxicity test, hemolysis assay, blood biochemistry test and histological staining. Results: The obtained nanodots show bright red fluorescence and highly effective 1O2 generation in aggregate state. Both in vitro and in vivo experiments demonstrate that the nanodots exhibit excellent tumor-targeted imaging performance, which facilitates image-guided PDT for tumor ablation in a hepatocellular carcinoma model. Detailed analysis reveals that the nanodot-mediated PDT is able to induce time- and concentration-dependent cell death. The use of PDT at a high PDT intensity leads to direct cell necrosis, while cell apoptosis via the mitochondria-mediated pathway is achieved under low PDT intensity. Conclusion: Our results suggest that well-designed AIE nanodots are promising for image-guided PDT applications. © Ivyspring International Publisher.
Source Title: Theranostics
URI: https://scholarbank.nus.edu.sg/handle/10635/213277
ISSN: 1838-7640
DOI: 10.7150/thno.29101
Rights: Attribution-NonCommercial 4.0 International
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