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Title: SERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian cancer
Authors: Perumal, J.
Mahyuddin, A.P. 
Balasundaram, G.
Goh, D.
Fu, C.Y.
Kazakeviciute, A.
Dinish, U.S.
Choolani, M. 
Olivo, M.
Keywords: Epithelial ovarian cancer
Ovarian cyst fluid
Point-of-care diagnostics
Surface-enhanced Raman spectroscopy
Issue Date: 2019
Publisher: Dove Medical Press Ltd
Citation: Perumal, J., Mahyuddin, A.P., Balasundaram, G., Goh, D., Fu, C.Y., Kazakeviciute, A., Dinish, U.S., Choolani, M., Olivo, M. (2019). SERS-based detection of haptoglobin in ovarian cyst fluid as a point-of-care diagnostic assay for epithelial ovarian cancer. Cancer Management and Research 11 : 1115-1124. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial 4.0 International
Abstract: Purpose: To evaluate haptoglobin (Hp) in ovarian cyst fluid as a diagnostic biomarker for epithelial ovarian cancers (EOCs) using surface-enhanced Raman spectroscopy (SERS)-based in vitro diagnostic assay for use in an intraoperative setting. Methods: SERS-based method was used to detect and quantify Hp in archived ovarian cyst fluids collected from suspicious ovarian cysts and differentiate benign tumors from EOCs. The diagnostic performance of SERS-based assay was verified against the histopathology conclusions and compared with the results of CA125 test and frozen sections. Results: Hp concentration present in the clinical cyst fluid measured by SERS was normalized to 3.3 mg/mL of standard Hp. Normalized mean values for patients with benign cysts were 0.65 (n=57) and malignant cysts were 1.85 (n=54), demonstrating a significantly (P<0.01) higher Hp in malignant samples. Verified against histology, Hp measurements using SERS had a sensitivity of 94% and specificity of 91%. Receiver operating characteristic curve analysis of SERS-based Hp measurements resulted in area under the curve of 0.966±0.03, establishing the robustness of the method. CA125 test on the same set of patients had a sensitivity of 85% and specificity of 90%, while frozen section analysis on 65 samples had 100% sensitivity and specificity. Conclusion: With a total execution time of <10 minutes and consistent performance across different stages of cancer, the SERS-based Hp detection assay can serve as a promising intraoperative EOC diagnostic test. © 2019 Perumal et al.
Source Title: Cancer Management and Research
ISSN: 1179-1322
DOI: 10.2147/CMAR.S185375
Rights: Attribution-NonCommercial 4.0 International
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