Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12870-019-2062-x
DC FieldValue
dc.titleA practical genome-enabled legitimacy assay for oil palm breeding and seed production
dc.contributor.authorTeh, C.-K.
dc.contributor.authorLee, H.-L.
dc.contributor.authorAbidin, H.
dc.contributor.authorOng, A.-L.
dc.contributor.authorMayes, S.
dc.contributor.authorChew, F.-T.
dc.contributor.authorAppleton, D.
dc.date.accessioned2022-01-07T03:50:47Z
dc.date.available2022-01-07T03:50:47Z
dc.date.issued2019
dc.identifier.citationTeh, C.-K., Lee, H.-L., Abidin, H., Ong, A.-L., Mayes, S., Chew, F.-T., Appleton, D. (2019). A practical genome-enabled legitimacy assay for oil palm breeding and seed production. BMC Plant Biology 19 (1) : 470. ScholarBank@NUS Repository. https://doi.org/10.1186/s12870-019-2062-x
dc.identifier.issn1471-2229
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/213250
dc.description.abstractBackground: Legitimacy in breeding and commercial crop production depends on optimised protocols to ensure purity of crosses and correct field planting of material. In oil palm, the presence of three fruit forms permits these assumptions to be tested, although only after field planting. The presence of incorrect fruit forms in a cross is a clear sign of illegitimacy. Given that tenera forms produce 30% more oil for the same weight of fruit as dura, the presence of low levels of dura contamination can have major effect during the economic lifespan of an oil palm, which is around 25 years. We evaluated two methods for legitimacy test 1) The use of SHELL markers to the gene that determines the shell-thickness trait 2) The use of SNP markers, to determine the legitimacy of the cross. Results: Our results indicate that the SHELL markers can theoretically reduce the major losses due to dura contamination of tenera planting material. However, these markers cannot distinguish illegitimate tenera, which reduces the value of having bred elite tenera for commercial planting and in the breeding programme, where fruit form is of limited utility, and incorrect identity could lead to significant problems. We propose an optimised approach using SNPs for routine quality control. Conclusions: Both dura and tenera contamination can be identified and removed at or before the nursery stage. An optimised legitimacy assay using SNP markers coupled with a suitable sampling scheme is now ready to be deployed as a standard control for seed production and breeding in oil palm. The same approach will also be an effective solution for other perennial crops, such as coconut and date palm. © 2019 The Author(s).
dc.publisherBioMed Central Ltd.
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2019
dc.subjectContamination
dc.subjectDNA fingerprinting
dc.subjectGenetic purity
dc.subjectSeed quality control
dc.subjectSHELL
dc.typeArticle
dc.contributor.departmentDEAN'S OFFICE (SCIENCE)
dc.description.doi10.1186/s12870-019-2062-x
dc.description.sourcetitleBMC Plant Biology
dc.description.volume19
dc.description.issue1
dc.description.page470
dc.published.statePublished
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1186_s12870-019-2062-x.pdf935.46 kBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

6
checked on Dec 2, 2022

Page view(s)

65
checked on Dec 1, 2022

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons