Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-019-10964-w
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dc.titleMyosin IIA and formin dependent mechanosensitivity of filopodia adhesion
dc.contributor.authorAlieva, N.O.
dc.contributor.authorEfremov, A.K.
dc.contributor.authorHu, S.
dc.contributor.authorOh, D.
dc.contributor.authorChen, Z.
dc.contributor.authorNatarajan, M.
dc.contributor.authorOng, H.T.
dc.contributor.authorJégou, A.
dc.contributor.authorRomet-Lemonne, G.
dc.contributor.authorGroves, J.T.
dc.contributor.authorSheetz, M.P.
dc.contributor.authorYan, J.
dc.contributor.authorBershadsky, A.D.
dc.date.accessioned2022-01-07T03:49:47Z
dc.date.available2022-01-07T03:49:47Z
dc.date.issued2019
dc.identifier.citationAlieva, N.O., Efremov, A.K., Hu, S., Oh, D., Chen, Z., Natarajan, M., Ong, H.T., Jégou, A., Romet-Lemonne, G., Groves, J.T., Sheetz, M.P., Yan, J., Bershadsky, A.D. (2019). Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion. Nature Communications 10 (1) : 3593. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-10964-w
dc.identifier.issn2041-1723
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/213240
dc.description.abstractFilopodia, dynamic membrane protrusions driven by polymerization of an actin filament core, can adhere to the extracellular matrix and experience both external and cell-generated pulling forces. The role of such forces in filopodia adhesion is however insufficiently understood. Here, we study filopodia induced by overexpression of myosin X, typical for cancer cells. The lifetime of such filopodia positively correlates with the presence of myosin IIA filaments at the filopodia bases. Application of pulling forces to the filopodia tips through attached fibronectin-coated laser-trapped beads results in sustained growth of the filopodia. Pharmacological inhibition or knockdown of myosin IIA abolishes the filopodia adhesion to the beads. Formin inhibitor SMIFH2, which causes detachment of actin filaments from formin molecules, produces similar effect. Thus, centripetal force generated by myosin IIA filaments at the base of filopodium and transmitted to the tip through actin core in a formin-dependent fashion is required for filopodia adhesion. © 2019, The Author(s).
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2019
dc.typeArticle
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1038/s41467-019-10964-w
dc.description.sourcetitleNature Communications
dc.description.volume10
dc.description.issue1
dc.description.page3593
dc.published.statePublished
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