Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/212695
Title: THE INTERPLAY OF PFKP AND AMPK IN METABOLIC STRESS IN LUNG CANCER
Authors: CHEN JIAQING
Keywords: PFKP, AMPK, metabolism, lung cancer
Issue Date: 18-Aug-2021
Citation: CHEN JIAQING (2021-08-18). THE INTERPLAY OF PFKP AND AMPK IN METABOLIC STRESS IN LUNG CANCER. ScholarBank@NUS Repository.
Abstract: Cancer cells adopt flexible metabolic phenotypes to promote cell survival under metabolic stress conditions. A key regulator of cell metabolism is AMP-activated kinase (AMPK) which promotes catabolic while suppresses anabolic metabolism. However, the function of AMPK in metabolic stress in cancer cells remains largely undefined. In this study, we performed a proteomics screening of AMPK-interacting proteins in non-small-cell lung cancer (NSCLC) cells and discovered a glycolysis enzyme named the platelet isoform of phosphofructokinase 1 (PFKP). Importantly, PFKP was found to be highly expressed in lung adenocarcinoma and correlated with poor prognosis. We also demonstrated that the PFKP-AMPK interaction was greatly enhanced upon glucose starvation in NSCLC. Of note, this interaction promoted the mitochondrial recruitment of AMPK which subsequently phosphorylated acetyl-CoA carboxylase 2 to upregulate long-chain fatty acid oxidation. The consumption of long-chain fatty acids maintained the energy and redox balance, and thereafter promoted cancer cell survival.
URI: https://scholarbank.nus.edu.sg/handle/10635/212695
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