Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-018-30557-9
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dc.titleAutomated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice
dc.contributor.authorWadduwage, D.N.
dc.contributor.authorKay, J.
dc.contributor.authorSingh, V.R.
dc.contributor.authorKiraly, O.
dc.contributor.authorSukup-Jackson, M.R.
dc.contributor.authorRajapakse, J.
dc.contributor.authorEngelward, B.P.
dc.contributor.authorSo, P.T.C.
dc.date.accessioned2021-12-17T06:08:31Z
dc.date.available2021-12-17T06:08:31Z
dc.date.issued2018
dc.identifier.citationWadduwage, D.N., Kay, J., Singh, V.R., Kiraly, O., Sukup-Jackson, M.R., Rajapakse, J., Engelward, B.P., So, P.T.C. (2018). Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice. Scientific Reports 8 (1) : 12108. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-018-30557-9
dc.identifier.issn20452322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/210980
dc.description.abstractHomologous recombination (HR) events are key drivers of cancer-promoting mutations, and the ability to visualize these events in situ provides important information regarding mutant cell type, location, and clonal expansion. We have previously created the Rosa26Direct Repeat (RaDR) mouse model wherein HR at an integrated substrate gives rise to a fluorescent cell. To fully leverage this in situ approach, we need better ways to quantify rare fluorescent cells deep within tissues. Here, we present a robust, automated event quantification algorithm that uses image intensity and gradient features to detect fluorescent cells in deep tissue specimens. To analyze the performance of our algorithm, we simulate fluorescence behavior in tissue using Monte Carlo methods. Importantly, this approach reduces the potential for bias in manual counting and enables quantification of samples with highly dense HR events. Using this approach, we measured the relative frequency of HR within a chromosome and between chromosomes and found that HR within a chromosome is more frequent, which is consistent with the close proximity of sister chromatids. Our approach is both objective and highly rapid, providing a powerful tool, not only to researchers interested in HR, but also to many other researchers who are similarly using fluorescence as a marker for understanding mammalian biology in tissues. © 2018, The Author(s).
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2018
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.1038/s41598-018-30557-9
dc.description.sourcetitleScientific Reports
dc.description.volume8
dc.description.issue1
dc.description.page12108
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