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Title: Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides
Keywords: Transcriptome,Three-finger toxins, proteome, tandem mass spectrometry, neurotoxin, genome walking
Issue Date: 12-Aug-2010
Citation: CHATRATH SHIFALI (2010-08-12). Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides. ScholarBank@NUS Repository.
Abstract: The venom composition of Drysdalia coronoides was elucidated by combined transcriptomics and proteomics approach. Three novel proteins, belonging to three-finger toxin superfamily of the snake venom proteins, were identified. One of them, named `drysdalin?, was highly expressed in the venom gland and was predicted to have unique structural changes related to its sequence. Drysdalin was recombinantly expressed in E. coli and its functional characterization was carried out by in vivo, ex vivo and in vitro assays. Drysdalin was found to be a post-synaptically acting neurotoxin and an irreversible antagonist of both neuronal as well as muscle type nicotinic acetylcholine receptor. We also attempted to determine the three dimensional structure of drysdalin. The results from the study have provided directions towards better understanding of the structure-function relationship of drysdalin.
Appears in Collections:Ph.D Theses (Open)

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