Please use this identifier to cite or link to this item: https://doi.org/10.3390/mps1020016
Title: Crispr-cas9 mediated genome editing in bicyclus anynana butterflies
Authors: Banerjee, T.D. 
Monteiro, A. 
Keywords: Bicyclus anynana
CRISPR-Cas9
SgRNA
Issue Date: 2018
Publisher: MDPI AG
Citation: Banerjee, T.D., Monteiro, A. (2018). Crispr-cas9 mediated genome editing in bicyclus anynana butterflies. Methods and Protocols 1 (2) : Jan-33. ScholarBank@NUS Repository. https://doi.org/10.3390/mps1020016
Rights: Attribution 4.0 International
Abstract: CRISPR-Cas9 is revolutionizing the field of genome editing in non-model organisms. The robustness, ease of use, replicability and affordability of the technology has resulted in its widespread adoption among researchers. The African butterfly Bicyclus anynana is an emerging model lepidopteran species in the field of evo-devo, with a sequenced genome and amenable to germ line transformation. However, efficient genome editing tools to accelerate the pace of functional genetic research in this species have only recently become available with CRISPR-Cas9 technology. Here, we provide a detailed explanation of the CRISPR-Cas9 protocol we follow in the lab. The technique has been successfully implemented to knock-out genes associated with eyespot development and melanin pigmentation. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Methods and Protocols
URI: https://scholarbank.nus.edu.sg/handle/10635/210102
ISSN: 2409-9279
DOI: 10.3390/mps1020016
Rights: Attribution 4.0 International
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