Please use this identifier to cite or link to this item:
https://doi.org/10.1093/nar/gkz717
Title: | DNA Analysis by Restriction Enzyme (DARE) enables concurrent genomic and epigenomic characterization of single cells | Authors: | Viswanathan, R. Cheruba, E. Cheow, L.F. |
Issue Date: | 2019 | Publisher: | NLM (Medline) | Citation: | Viswanathan, R., Cheruba, E., Cheow, L.F. (2019). DNA Analysis by Restriction Enzyme (DARE) enables concurrent genomic and epigenomic characterization of single cells. Nucleic acids research 47 (19) : e122. ScholarBank@NUS Repository. https://doi.org/10.1093/nar/gkz717 | Rights: | Attribution-NonCommercial 4.0 International | Abstract: | Genome-wide profiling of copy number alterations and DNA methylation in single cells could enable detailed investigation into the genomic and epigenomic heterogeneity of complex cell populations. However, current methods to do this require complex sample processing and cleanup steps, lack consistency, or are biased in their genomic representation. Here, we describe a novel single-tube enzymatic method, DNA Analysis by Restriction Enzyme (DARE), to perform deterministic whole genome amplification while preserving DNA methylation information. This method was evaluated on low amounts of DNA and single cells, and provides accurate copy number aberration calling and representative DNA methylation measurement across the whole genome. Single-cell DARE is an attractive and scalable approach for concurrent genomic and epigenomic characterization of cells in a heterogeneous population. © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. | Source Title: | Nucleic acids research | URI: | https://scholarbank.nus.edu.sg/handle/10635/209533 | ISSN: | 1362-4962 | DOI: | 10.1093/nar/gkz717 | Rights: | Attribution-NonCommercial 4.0 International |
Appears in Collections: | Staff Publications Elements |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1093_nar_gkz717.pdf | 6.73 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License