Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/209458
DC Field | Value | |
---|---|---|
dc.title | SYNTHETIC CANNABINOID BIOLOGY | |
dc.contributor.author | KEVIN LIM JIE HAN | |
dc.date.accessioned | 2021-12-03T18:00:27Z | |
dc.date.available | 2021-12-03T18:00:27Z | |
dc.date.issued | 2021-07-23 | |
dc.identifier.citation | KEVIN LIM JIE HAN (2021-07-23). SYNTHETIC CANNABINOID BIOLOGY. ScholarBank@NUS Repository. | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/209458 | |
dc.description.abstract | There is increasing demand around the world for cannabinoids and their derivatives due to their potential in therapeutics and nutraceuticals leading to a growing concern for environmental and social problems associated with cannabis cultivation. Synthetic biology efforts for cannabinoid biosynthesis have been explored to address these challenges. Here, we reconstructed the entire cannabinoid biosynthetic pathway in microbial cell factories to provide a biotechnologically engineered and tractable way of producing specific cannabinoids of interest from simple precursors. Additionally, we enlisted the use of computational tools to establish a generalizable computational workflow that aided in overcoming a significant bottleneck in the biosynthetic pathway through computational protein engineering. The working cannabinoid-producing strain established here provided access to the biosynthesis of a bespoke combinatorial library of rare and/or novel cannabinoids. We extensively explored avenues of producing these cannabinoid analogues to create a combinatorial library of rare and/or novel cannabinoids for future drug discovery studies. | |
dc.language.iso | en | |
dc.subject | Cannabis sativa, cannabinoids biosynthesis, metabolic engineering; synthetic biology, protein engineering, drug discovery | |
dc.type | Thesis | |
dc.contributor.department | DEAN'S OFFICE (MEDICINE) | |
dc.contributor.supervisor | Wen Shan Yew | |
dc.contributor.supervisor | Hao Fan | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY (SOM) | |
dc.identifier.orcid | 0000-0002-0356-4093 | |
Appears in Collections: | Ph.D Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
LimKJH_Synthetic_Cannabinoid_Biology.pdf | 6.84 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.