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Title: Gene expression changes in the brainstem in a mouse model of orofacial pain
Keywords: gene expression change, orofacial pain, microarrays analysis, genes regulation in brainstem
Issue Date: 21-Aug-2009
Citation: LUTFUN NAHAR (2009-08-21). Gene expression changes in the brainstem in a mouse model of orofacial pain. ScholarBank@NUS Repository.
Abstract: The present study was carried out to examine possible gene expression changes that occur in the brainstem in a mouse facial carrageenan injection model of orofacial pain. Mice that received facial carrageenan injection showed increased mechanical allodynia, demonstrated by increased responses to von Frey hair stimulation of the face. The brainstem was harvested at 3 days post-injection, corresponding to the time of peak responses, and analyzed by Affymetrix Mouse Genome 430 2.0 microarrays. Large number of genes were up or down regulated in the brainstem after carrageenan injection, but the number of genes that showed common change after right or left sided facial carrageenan injection were relatively small. The common genes were then classified and analysed by using Database for Annotation, Visualization, and Integrated Discovery (DAVID) software (Dennis et al., 2003). Most of them were upregulated and the largest group of genes was in the category of ?host defence genes against pathogens?. These include chemokine, inflammation related, and endothelial related genes. Of these, increased expression of P-selectin, ICAM-1 and CCL12 after carrageenan injection could be verified by real-time RT-PCR on both the right and left sides, and the increases in P-selectin and ICAM-1 further verified by Western blot analysis and immunohistochemistry. CCL12 is closely related to human MCP-1/CCL2 in structure and may contribute to a signalling system that might cause neuronal hyperexcitability. ICAM-1 is an immunoglobulin like cell adhesion molecule that binds to leukocytes. It recruits immunocytes containing opioids to facilitate the local control of inflammatory pain. P-selectin is a marker for platelet activation and endothelial dysfunction. P-selectin mediates the capturing of leukocytes from the blood stream and rolling of leukocytes along the endothelial surface. It is hypothesize that increased nociceptive input to the brainstem could attract circulating macrophages into the brain, resulting in neuroinflammation and pain. The present findings suggest that CCL12, ICAM-1, and P-selectin may play a role in orofacial pain.
Appears in Collections:Master's Theses (Open)

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