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Title: Development of microplate screening system and construction of fluorescein-based library
Keywords: fluorescence, library, cell imaging, chemical biology, fluorescein,
Issue Date: 10-Aug-2010
Citation: FENG SUIHAN (2010-08-10). Development of microplate screening system and construction of fluorescein-based library. ScholarBank@NUS Repository.
Abstract: Chemical genetics studies the structures and functions of genes at the molecular level using small molecules. Probe development is thus of great interests in molecular biology, particularly in cell imaging study. Due to the simplicity and straight forwardness, fluorescence techniques are emerging recently to help elucidate and explain complicated biological phenomena in cells and animals. Furthermore, the green fluorescein protein (GFP) also facilitated the progress of fluorescence imaging research. However, novel fluorescent sensor design is still difficult and time consuming, as little knowledge is well understood in this field. There is a pressing need to development new approaches to design fluorescent sensors. Diversity Oriented Fluorescence Library Approach (DOFLA) provided an alternative strategy to generate large number of fluorescent compounds by combining solid phase chemistry and combinatorial chemistry. Although the obstacle of generating diversified fluorophores could be achieved, it is crucial to design a practical platform to select fluorescent sensors. Chapter 2 describes a novel biological screening system to find potential sensors. Besides, one green DNA probe, C61, to stain live and dead cells is presented. Compare with available green fluorescent sensors, C61 showed dramatic DNA selectivity over RNA. Chapter 3 presents one chloro-fluorescein library synthesis and its biological exploration in melanoma and skill cancer cells.
Appears in Collections:Master's Theses (Open)

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