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Title: Tissue Engineering Approach for Annulus Fibrosus Regeneration
Keywords: Tissue Engineering, Intervertebral Disc, Annulus Fibrosus, Bone Marrow Stem Cells, Cell-Sheet
Issue Date: 24-Sep-2010
Citation: SEE YONG-SHUN, EUGENE (2010-09-24). Tissue Engineering Approach for Annulus Fibrosus Regeneration. ScholarBank@NUS Repository.
Abstract: The aim of this study was to develop a tissue engineering approach in regenerating the annulus fibrosus (AF) as part of an overall strategy to produce a tissue-engineered intervertebral disc replacement. The approach was to use bone marrow derived stem cells (BMSC) to form cell-sheets and incorporating them onto silk scaffolds to simulate the native lamellae of the AF. The in vitro experimental model used to study the efficacy of such a system was made up of the tissue engineering AF construct wrapped around a silicone disc to form a simulated IVD-like assembly. The AF construct was cultured within a custom-designed bioreactor that provided a mechanical stimulation to mimic the physiological condition. The results showed that BMSC cell-sheets retain their multipotentiality and were a suitable cell source for the simulated AF. The use of the bioreactor on the experimental model was shown to further enhance the efficacy in regenerating the inner AF.
Appears in Collections:Ph.D Theses (Open)

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