Please use this identifier to cite or link to this item: https://doi.org/10.1002/adbi.201700217
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dc.titleEvaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells
dc.contributor.authorKukumberg, Marek
dc.contributor.authorYao, Yuan
dc.contributor.authorGoh, Seok Hong
dc.contributor.authorNeo, Dawn JH
dc.contributor.authorYao, Jia Yi
dc.contributor.authorYim, Evelyn KF
dc.date.accessioned2021-11-24T01:57:24Z
dc.date.available2021-11-24T01:57:24Z
dc.date.issued2018-06-01
dc.identifier.citationKukumberg, Marek, Yao, Yuan, Goh, Seok Hong, Neo, Dawn JH, Yao, Jia Yi, Yim, Evelyn KF (2018-06-01). Evaluation of the Topographical Influence on the Cellular Behavior of Human Umbilical Vein Endothelial Cells. ADVANCED BIOSYSTEMS 2 (6). ScholarBank@NUS Repository. https://doi.org/10.1002/adbi.201700217
dc.identifier.issn23667478
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/207658
dc.description.abstractAdhesion and proliferation of vascular endothelial cells are important parameters in the endothelialization of biomedical devices for vascular applications. Endothelialization is a complex process affected by endothelial cells and their interaction with the extracellular microenvironment. Although numerous approaches are taken to study the influence of the external environment, a systematic investigation of the impact of an engineered microenvironment on endothelial cell processes is needed. This study aims to investigate the influence of topography, initial cell seeding density, and collagen coating on human umbilical vein endothelial cells (HUVECs). Utilizing the MultiARChitecture (MARC) chamber, the effects of various topographies on HUVECs are identified, and those with more prominent effects were further evaluated individually using the MARC plate. Endothelial cell marker expression and monocyte adhesion assay are examined on the HUVEC monolayer. HUVECs on 1.8 μm convex and concave microlens topographies demonstrate the lowest cell adhesion and proliferation, regardless of initial cell seeding density and collagen I coating, and the HUVEC monolayer on the microlens shows the lowest monocyte adhesion. This property of lens topographies would potentially be a useful parameter in designing vascular biomedical devices. The MARC chamber and MARC plate show a great potential for faster and easy pattern identification for various cellular processes.
dc.language.isoen
dc.publisherWILEY-V C H VERLAG GMBH
dc.sourceElements
dc.subjectScience & Technology
dc.subjectTechnology
dc.subjectMaterials Science, Biomaterials
dc.subjectMaterials Science
dc.subjectendothelial cells
dc.subjectextracellular matrix coating
dc.subjecthigh-throughput screening
dc.subjectmicrotopography
dc.subjectmonocytes adhesion assay
dc.subjectproliferation
dc.subjectSHEAR-STRESS
dc.subjectEXTRACELLULAR-MATRIX
dc.subjectMONOCYTE ADHESION
dc.subjectSTEM-CELLS
dc.subjectSUBSTRATE TOPOGRAPHY
dc.subjectNANO-TOPOGRAPHIES
dc.subjectBASEMENT-MEMBRANE
dc.subjectSEEDING DENSITY
dc.subjectIN-VITRO
dc.subjectMICRO
dc.typeArticle
dc.date.updated2021-11-22T02:33:25Z
dc.contributor.departmentDEPT OF SURGERY
dc.description.doi10.1002/adbi.201700217
dc.description.sourcetitleADVANCED BIOSYSTEMS
dc.description.volume2
dc.description.issue6
dc.published.statePublished
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