Please use this identifier to cite or link to this item: https://doi.org/10.1111/aos.12247
Title: Indocyanine green concentrations used in chromovitrectomy cause a reversible functional alteration in the outer blood-retinal barrier
Authors: LIU ZENGPING 
Meyer, Carsten H
Fimmers, Rolf
Stanzel, Boris V
Keywords: Science & Technology
Life Sciences & Biomedicine
Ophthalmology
tight junctions
retinal pigment epithelium
chromovitrectomy
transepithelial electrical resistance
indocyanine green
cell culture
blood-retinal barrier
PIGMENT EPITHELIAL-CELLS
MACULAR HOLE SURGERY
BRILLIANT BLUE-G
INTERNAL LIMITING MEMBRANE
TIGHT JUNCTIONS
TRYPAN BLUE
VITAL DYES
IN-VITRO
EPIRETINAL MEMBRANE
PERMEABILITY
Issue Date: 1-Mar-2014
Publisher: WILEY-BLACKWELL
Citation: LIU ZENGPING, Meyer, Carsten H, Fimmers, Rolf, Stanzel, Boris V (2014-03-01). Indocyanine green concentrations used in chromovitrectomy cause a reversible functional alteration in the outer blood-retinal barrier. ACTA OPHTHALMOLOGICA 92 (2) : E147-E155. ScholarBank@NUS Repository. https://doi.org/10.1111/aos.12247
Abstract: PURPOSE: To assess tight junction integrity in cultured human foetal retinal pigment epithelium (RPE) after exposure to clinically relevant indocyanine green (ICG) concentrations. METHODS: Human foetal RPE was cultured with the Hu & Bok method. The apical compartments of well-differentiated cultures were exposed to 0.125, 0.05 and 0.025 mg/ml ICG with or without 10-min illumination. Vehicle and trypsin/EDTA or EDTA alone served as controls. Three minutes was chosen to mimic surgical exposure time, while 3 h was used for toxicity assays, with subsequent wash out. Cell-cell junctions were studied before and after exposure by phase contrast microscopy and immunofluorescence (ZO-1). Blood-retinal barrier function was measured through transepithelial electrical resistance (TER). RESULTS: At 6-8 weeks postconfluence, RPE had grown into pigmented hexagonal monolayers with stable TER (435-1227 Ω*cm(2) ). After 3 min ICG exposure, cell morphology remained unchanged, with patchy cell-cell dissociation in positive controls. A continuous ZO-1 signal was detected in ICG groups, whereas trypsin controls showed patchy loss of the tight junction stain. TER had dropped at 1.5 h after 3 min exposure to 22.8 ± 3.1%, compared with 10.2 ± 3.9% in positive controls. Surgical light illumination did not affect TER. After 3 h exposure to 0.05 mg/ml ICG, TER decreased to 58.1 ± 8.3%, while vehicle controls maintained similar levels as prior to exposure (92.7 ± 2.4%). TER recovered in all ICG groups to prior levels within 3 days. CONCLUSION: Indocyanine green (ICG) exposure induced a transient decrease in transepithelial electrical resistance, despite unaltered tight junction structure.
Source Title: ACTA OPHTHALMOLOGICA
URI: https://scholarbank.nus.edu.sg/handle/10635/206756
ISSN: 1755-375X
1755-3768
DOI: 10.1111/aos.12247
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