Please use this identifier to cite or link to this item: https://doi.org/10.1111/aos.12247
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dc.titleIndocyanine green concentrations used in chromovitrectomy cause a reversible functional alteration in the outer blood-retinal barrier
dc.contributor.authorLIU ZENGPING
dc.contributor.authorMeyer, Carsten H
dc.contributor.authorFimmers, Rolf
dc.contributor.authorStanzel, Boris V
dc.date.accessioned2021-11-19T04:11:09Z
dc.date.available2021-11-19T04:11:09Z
dc.date.issued2014-03-01
dc.identifier.citationLIU ZENGPING, Meyer, Carsten H, Fimmers, Rolf, Stanzel, Boris V (2014-03-01). Indocyanine green concentrations used in chromovitrectomy cause a reversible functional alteration in the outer blood-retinal barrier. ACTA OPHTHALMOLOGICA 92 (2) : E147-E155. ScholarBank@NUS Repository. https://doi.org/10.1111/aos.12247
dc.identifier.issn1755-375X
dc.identifier.issn1755-3768
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/206756
dc.description.abstractPURPOSE: To assess tight junction integrity in cultured human foetal retinal pigment epithelium (RPE) after exposure to clinically relevant indocyanine green (ICG) concentrations. METHODS: Human foetal RPE was cultured with the Hu & Bok method. The apical compartments of well-differentiated cultures were exposed to 0.125, 0.05 and 0.025 mg/ml ICG with or without 10-min illumination. Vehicle and trypsin/EDTA or EDTA alone served as controls. Three minutes was chosen to mimic surgical exposure time, while 3 h was used for toxicity assays, with subsequent wash out. Cell-cell junctions were studied before and after exposure by phase contrast microscopy and immunofluorescence (ZO-1). Blood-retinal barrier function was measured through transepithelial electrical resistance (TER). RESULTS: At 6-8 weeks postconfluence, RPE had grown into pigmented hexagonal monolayers with stable TER (435-1227 Ω*cm(2) ). After 3 min ICG exposure, cell morphology remained unchanged, with patchy cell-cell dissociation in positive controls. A continuous ZO-1 signal was detected in ICG groups, whereas trypsin controls showed patchy loss of the tight junction stain. TER had dropped at 1.5 h after 3 min exposure to 22.8 ± 3.1%, compared with 10.2 ± 3.9% in positive controls. Surgical light illumination did not affect TER. After 3 h exposure to 0.05 mg/ml ICG, TER decreased to 58.1 ± 8.3%, while vehicle controls maintained similar levels as prior to exposure (92.7 ± 2.4%). TER recovered in all ICG groups to prior levels within 3 days. CONCLUSION: Indocyanine green (ICG) exposure induced a transient decrease in transepithelial electrical resistance, despite unaltered tight junction structure.
dc.language.isoen
dc.publisherWILEY-BLACKWELL
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectOphthalmology
dc.subjecttight junctions
dc.subjectretinal pigment epithelium
dc.subjectchromovitrectomy
dc.subjecttransepithelial electrical resistance
dc.subjectindocyanine green
dc.subjectcell culture
dc.subjectblood-retinal barrier
dc.subjectPIGMENT EPITHELIAL-CELLS
dc.subjectMACULAR HOLE SURGERY
dc.subjectBRILLIANT BLUE-G
dc.subjectINTERNAL LIMITING MEMBRANE
dc.subjectTIGHT JUNCTIONS
dc.subjectTRYPAN BLUE
dc.subjectVITAL DYES
dc.subjectIN-VITRO
dc.subjectEPIRETINAL MEMBRANE
dc.subjectPERMEABILITY
dc.typeArticle
dc.date.updated2021-11-18T15:25:32Z
dc.contributor.departmentDEPT OF OPHTHALMOLOGY
dc.description.doi10.1111/aos.12247
dc.description.sourcetitleACTA OPHTHALMOLOGICA
dc.description.volume92
dc.description.issue2
dc.description.pageE147-E155
dc.published.statePublished
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