Please use this identifier to cite or link to this item: https://doi.org/10.1159/000353869
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dc.titleEffect of Novel Vital Dyes on Outer Blood-Retina Barrier Function in Cultured Human Retinal Pigment Epithelium
dc.contributor.authorLIU ZENGPING
dc.contributor.authorMeyer, Carsten H
dc.contributor.authorStanzel, Boris V
dc.date.accessioned2021-11-19T03:53:44Z
dc.date.available2021-11-19T03:53:44Z
dc.date.issued2013-01-01
dc.identifier.citationLIU ZENGPING, Meyer, Carsten H, Stanzel, Boris V (2013-01-01). Effect of Novel Vital Dyes on Outer Blood-Retina Barrier Function in Cultured Human Retinal Pigment Epithelium. OPHTHALMOLOGICA 230 (s2) : 33-40. ScholarBank@NUS Repository. https://doi.org/10.1159/000353869
dc.identifier.issn0030-3755
dc.identifier.issn1423-0267
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/206752
dc.description.abstractPURPOSE: To assess tight junction (TJ) integrity in cultured human fetal retinal pigment epithelium (HFRPE) after exposure to clinically relevant novel vital dyes. METHODS: HFRPE floater cells were harvested from RPE primary cultures of 4 donor eyes and seeded on polyester Transwell® for 4-6 weeks. The apical compartments of well-differentiated cultures were exposed to 0.005 mg/ml Coomassie violet R200 (CVR), methyl 2B (M2B) or Orange II. Periods of 30-300 s were chosen to mimic surgical exposure times, while 3 h was used for toxicity assays, with subsequent washout. Cell-cell junctions were studied by immunofluorescence (zonula occludens-1, ZO-1). Transepithelial electrical resistance (TER) was measured regarding blood-retina barrier (BRB) function. RESULTS: At 4-6 weeks after confluence, HFRPE had grown into pigmented hexagonal monolayers with stable TER values (451-1,520 Ω·cm(2)). After 300-second dye treatments, a continuous ZO-1 signal was detected in all vital dye-treated groups 1.5 h after exposure, whereas trypsin controls showed patchy loss of the TJ stain. TER of CVR-, M2B- and Orange-II-treated groups had dropped 1.5 h after exposure to 148 ± 58.4, 162 ± 23.7 and 164 ± 18.5 Ω·cm(2), respectively, compared to 73 ± 44.9 Ω·cm(2) in positive controls. After 3 h of exposure to 0.005 mg/ml vital dyes in thick drops, TER maintained similar levels to those prior to exposure (90.8 ± 4.7% of the original values, 93.8 ± 6.5 and 91.9 ± 3.6%, respectively), together with no difference from the vehicle controls (94.8 ± 6.6%). TER values recovered in all groups to prior levels within 3 days. CONCLUSION: Novel vital dyes (CVR, M2B and Orange II) caused no outer BRB function alteration.
dc.language.isoen
dc.publisherKARGER
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectOphthalmology
dc.subjectBlood-retina barrier
dc.subjectCell culture
dc.subjectChromovitrectomy
dc.subjectRetinal pigment epithelium
dc.subjectTight junctions
dc.subjectTransepithelial electrical resistance
dc.subjectINTERNAL LIMITING MEMBRANE
dc.subjectMACULAR HOLE SURGERY
dc.subjectINDOCYANINE GREEN
dc.subjectTIGHT JUNCTIONS
dc.subjectIN-VITRO
dc.subjectCELLS
dc.subjectPERMEABILITY
dc.subjectMONOLAYERS
dc.subjectMECHANISMS
dc.subjectSAFETY
dc.typeArticle
dc.date.updated2021-11-18T15:23:08Z
dc.contributor.departmentDEPT OF OPHTHALMOLOGY
dc.description.doi10.1159/000353869
dc.description.sourcetitleOPHTHALMOLOGICA
dc.description.volume230
dc.description.issues2
dc.description.page33-40
dc.published.statePublished
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