Please use this identifier to cite or link to this item:
https://doi.org/10.1159/000353869
DC Field | Value | |
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dc.title | Effect of Novel Vital Dyes on Outer Blood-Retina Barrier Function in Cultured Human Retinal Pigment Epithelium | |
dc.contributor.author | LIU ZENGPING | |
dc.contributor.author | Meyer, Carsten H | |
dc.contributor.author | Stanzel, Boris V | |
dc.date.accessioned | 2021-11-19T03:53:44Z | |
dc.date.available | 2021-11-19T03:53:44Z | |
dc.date.issued | 2013-01-01 | |
dc.identifier.citation | LIU ZENGPING, Meyer, Carsten H, Stanzel, Boris V (2013-01-01). Effect of Novel Vital Dyes on Outer Blood-Retina Barrier Function in Cultured Human Retinal Pigment Epithelium. OPHTHALMOLOGICA 230 (s2) : 33-40. ScholarBank@NUS Repository. https://doi.org/10.1159/000353869 | |
dc.identifier.issn | 0030-3755 | |
dc.identifier.issn | 1423-0267 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/206752 | |
dc.description.abstract | PURPOSE: To assess tight junction (TJ) integrity in cultured human fetal retinal pigment epithelium (HFRPE) after exposure to clinically relevant novel vital dyes. METHODS: HFRPE floater cells were harvested from RPE primary cultures of 4 donor eyes and seeded on polyester Transwell® for 4-6 weeks. The apical compartments of well-differentiated cultures were exposed to 0.005 mg/ml Coomassie violet R200 (CVR), methyl 2B (M2B) or Orange II. Periods of 30-300 s were chosen to mimic surgical exposure times, while 3 h was used for toxicity assays, with subsequent washout. Cell-cell junctions were studied by immunofluorescence (zonula occludens-1, ZO-1). Transepithelial electrical resistance (TER) was measured regarding blood-retina barrier (BRB) function. RESULTS: At 4-6 weeks after confluence, HFRPE had grown into pigmented hexagonal monolayers with stable TER values (451-1,520 Ω·cm(2)). After 300-second dye treatments, a continuous ZO-1 signal was detected in all vital dye-treated groups 1.5 h after exposure, whereas trypsin controls showed patchy loss of the TJ stain. TER of CVR-, M2B- and Orange-II-treated groups had dropped 1.5 h after exposure to 148 ± 58.4, 162 ± 23.7 and 164 ± 18.5 Ω·cm(2), respectively, compared to 73 ± 44.9 Ω·cm(2) in positive controls. After 3 h of exposure to 0.005 mg/ml vital dyes in thick drops, TER maintained similar levels to those prior to exposure (90.8 ± 4.7% of the original values, 93.8 ± 6.5 and 91.9 ± 3.6%, respectively), together with no difference from the vehicle controls (94.8 ± 6.6%). TER values recovered in all groups to prior levels within 3 days. CONCLUSION: Novel vital dyes (CVR, M2B and Orange II) caused no outer BRB function alteration. | |
dc.language.iso | en | |
dc.publisher | KARGER | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Ophthalmology | |
dc.subject | Blood-retina barrier | |
dc.subject | Cell culture | |
dc.subject | Chromovitrectomy | |
dc.subject | Retinal pigment epithelium | |
dc.subject | Tight junctions | |
dc.subject | Transepithelial electrical resistance | |
dc.subject | INTERNAL LIMITING MEMBRANE | |
dc.subject | MACULAR HOLE SURGERY | |
dc.subject | INDOCYANINE GREEN | |
dc.subject | TIGHT JUNCTIONS | |
dc.subject | IN-VITRO | |
dc.subject | CELLS | |
dc.subject | PERMEABILITY | |
dc.subject | MONOLAYERS | |
dc.subject | MECHANISMS | |
dc.subject | SAFETY | |
dc.type | Article | |
dc.date.updated | 2021-11-18T15:23:08Z | |
dc.contributor.department | DEPT OF OPHTHALMOLOGY | |
dc.description.doi | 10.1159/000353869 | |
dc.description.sourcetitle | OPHTHALMOLOGICA | |
dc.description.volume | 230 | |
dc.description.issue | s2 | |
dc.description.page | 33-40 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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P8- Liu Ophthalmologica 2013.pdf | 251.17 kB | Adobe PDF | CLOSED | Published |
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