Please use this identifier to cite or link to this item:
https://doi.org/10.3390/cancers13020340
DC Field | Value | |
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dc.title | T-Cell Lymphoma Clonality by Copy Number Variation Analysis of T-Cell Receptor Genes | |
dc.contributor.author | Oon, Ming Liang | |
dc.contributor.author | Lim, Jing Quan | |
dc.contributor.author | Lee, Bernett | |
dc.contributor.author | Leong, Sai Mun | |
dc.contributor.author | Soon, Gwyneth Shook-Ting | |
dc.contributor.author | Wong, Zi Wei | |
dc.contributor.author | Lim, Evelyn Huizi | |
dc.contributor.author | Li, Zhenhua | |
dc.contributor.author | Yeoh, Allen Eng Juh | |
dc.contributor.author | Chen, Shangying | |
dc.contributor.author | Ban, Kenneth Hon Kim | |
dc.contributor.author | Chung, Tae-Hoon | |
dc.contributor.author | Tan, Soo-Yong | |
dc.contributor.author | Chuang, Shih-Sung | |
dc.contributor.author | Kato, Seiichi | |
dc.contributor.author | Nakamura, Shigeo | |
dc.contributor.author | Takahashi, Emiko | |
dc.contributor.author | Ho, Yong-Howe | |
dc.contributor.author | Khoury, Joseph D | |
dc.contributor.author | Au-Yeung, Rex KH | |
dc.contributor.author | Cheng, Chee-Leong | |
dc.contributor.author | Lim, Soon-Thye | |
dc.contributor.author | Chng, Wee-Joo | |
dc.contributor.author | Tripodo, Claudio | |
dc.contributor.author | Rotzschke, Olaf | |
dc.contributor.author | Ong, Choon Kiat | |
dc.contributor.author | Ng, Siok-Bian | |
dc.date.accessioned | 2021-11-17T09:05:27Z | |
dc.date.available | 2021-11-17T09:05:27Z | |
dc.date.issued | 2021-01-01 | |
dc.identifier.citation | Oon, Ming Liang, Lim, Jing Quan, Lee, Bernett, Leong, Sai Mun, Soon, Gwyneth Shook-Ting, Wong, Zi Wei, Lim, Evelyn Huizi, Li, Zhenhua, Yeoh, Allen Eng Juh, Chen, Shangying, Ban, Kenneth Hon Kim, Chung, Tae-Hoon, Tan, Soo-Yong, Chuang, Shih-Sung, Kato, Seiichi, Nakamura, Shigeo, Takahashi, Emiko, Ho, Yong-Howe, Khoury, Joseph D, Au-Yeung, Rex KH, Cheng, Chee-Leong, Lim, Soon-Thye, Chng, Wee-Joo, Tripodo, Claudio, Rotzschke, Olaf, Ong, Choon Kiat, Ng, Siok-Bian (2021-01-01). T-Cell Lymphoma Clonality by Copy Number Variation Analysis of T-Cell Receptor Genes. CANCERS 13 (2). ScholarBank@NUS Repository. https://doi.org/10.3390/cancers13020340 | |
dc.identifier.issn | 20726694 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/206597 | |
dc.description.abstract | T-cell lymphomas arise from a single neoplastic clone and exhibit identical patterns of deletions in T-cell receptor (TCR) genes. Whole genome sequencing (WGS) data represent a treasure trove of information for the development of novel clinical applications. However, the use of WGS to identify clonal T-cell proliferations has not been systematically studied. In this study, based on WGS data, we identified monoclonal rearrangements (MRs) of T-cell receptors (TCR) genes using a novel segmentation algorithm and copy number computation. We evaluated the feasibility of this technique as a marker of T-cell clonality using T-cell lymphomas (TCL, n = 44) and extranodal NK/T-cell lymphomas (ENKTLs, n = 20), and identified 98% of TCLs with one or more TCR gene MRs, against 91% detected using PCR. TCR MRs were absent in all ENKTLs and NK cell lines. Sensitivity-wise, this platform is sufficiently competent, with MRs detected in the majority of samples with tumor content under 25% and it can also distinguish monoallelic from biallelic MRs. Understanding the copy number landscape of TCR using WGS data may engender new diagnostic applications in hematolymphoid pathology, which can be readily adapted to the analysis of B-cell receptor loci for B-cell clonality determination. | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Oncology | |
dc.subject | whole genome sequencing | |
dc.subject | T-cell receptor | |
dc.subject | clonality | |
dc.subject | copy number variation analysis | |
dc.subject | T-cell lymphoma | |
dc.type | Article | |
dc.date.updated | 2021-11-17T06:41:24Z | |
dc.contributor.department | DEPT OF PATHOLOGY | |
dc.contributor.department | DEAN'S OFFICE (DUKE-NUS MEDICAL SCHOOL) | |
dc.contributor.department | PAEDIATRICS | |
dc.contributor.department | DEPT OF BIOCHEMISTRY | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.description.doi | 10.3390/cancers13020340 | |
dc.description.sourcetitle | CANCERS | |
dc.description.volume | 13 | |
dc.description.issue | 2 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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T cell clonality CNV Cancers 2021 Jan.pdf | Published version | 4.03 MB | Adobe PDF | OPEN | None | View/Download |
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