Please use this identifier to cite or link to this item: https://doi.org/10.3390/cells8090986
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dc.titleRNA Sequencing of H3N2 Influenza Virus-Infected Human Nasal Epithelial Cells from Multiple Subjects Reveals Molecular Pathways Associated with Tissue Injury and Complications
dc.contributor.authorTan, K.S.
dc.contributor.authorAndiappan, A.K.
dc.contributor.authorLee, B.
dc.contributor.authorYan, Y.
dc.contributor.authorLiu, J.
dc.contributor.authorTang, S.A.
dc.contributor.authorLum, J.
dc.contributor.authorHe, T.T.
dc.contributor.authorOng, Y.K.
dc.contributor.authorThong, M.
dc.contributor.authorLim, H.F.
dc.contributor.authorChoi, H.W.
dc.contributor.authorRotzschke, O.
dc.contributor.authorChow, V.T.
dc.contributor.authorWang, Y.
dc.date.accessioned2021-11-16T03:57:58Z
dc.date.available2021-11-16T03:57:58Z
dc.date.issued2019
dc.identifier.citationTan, K.S., Andiappan, A.K., Lee, B., Yan, Y., Liu, J., Tang, S.A., Lum, J., He, T.T., Ong, Y.K., Thong, M., Lim, H.F., Choi, H.W., Rotzschke, O., Chow, V.T., Wang, Y. (2019). RNA Sequencing of H3N2 Influenza Virus-Infected Human Nasal Epithelial Cells from Multiple Subjects Reveals Molecular Pathways Associated with Tissue Injury and Complications. Cells 8 (9). ScholarBank@NUS Repository. https://doi.org/10.3390/cells8090986
dc.identifier.issn2073-4409
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/206297
dc.description.abstractThe human nasal epithelium is the primary site of exposure to influenza virus, the initiator of host responses to influenza and the resultant pathologies. Influenza virus may cause serious respiratory infection resulting in major complications, as well as severe impairment of the airways. Here, we elucidated the global transcriptomic changes during H3N2 infection of human nasal epithelial cells from multiple individuals. Using RNA sequencing, we characterized the differentially-expressed genes and pathways associated with changes occurring at the nasal epithelium following infection. We used in vitro differentiated human nasal epithelial cell culture model derived from seven different donors who had no concurrent history of viral infections. Statistical analysis highlighted strong transcriptomic signatures significantly associated with 24 and 48 h after infection, but not at the earlier 8-h time point. In particular, we found that the influenza infection induced in the nasal epithelium early and altered responses in interferon gamma signaling, B-cell signaling, apoptosis, necrosis, smooth muscle proliferation, and metabolic alterations. These molecular events initiated at the infected nasal epithelium may potentially adversely impact the airway, and thus the genes we identified could serve as potential diagnostic biomarkers or therapeutic targets for influenza infection and associated disease management.
dc.publisherNLM (Medline)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2019
dc.subjectepithelial responses
dc.subjectH3N2 virus
dc.subjecthuman nasal epithelial cells (hNECs)
dc.subjectinfluenza
dc.subjectpathogenesis
dc.subjectRNA sequencing
dc.subjecttranscriptomics
dc.typeArticle
dc.contributor.departmentOTOLARYNGOLOGY
dc.contributor.departmentMEDICINE
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.description.doi10.3390/cells8090986
dc.description.sourcetitleCells
dc.description.volume8
dc.description.issue9
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