Please use this identifier to cite or link to this item: https://doi.org/10.3389/fbioe.2019.00266
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dc.title3D-Printed PCL/PPy Conductive Scaffolds as Three-Dimensional Porous Nerve Guide Conduits (NGCs) for Peripheral Nerve Injury Repair
dc.contributor.authorVijayavenkataraman, Sanjairaj
dc.contributor.authorKannan, Sathya
dc.contributor.authorCao, Tong
dc.contributor.authorFuh, Jerry YH
dc.contributor.authorSriram, Gopu
dc.contributor.authorLu, Wen Feng
dc.date.accessioned2021-11-11T09:11:23Z
dc.date.available2021-11-11T09:11:23Z
dc.date.issued2019-10-16
dc.identifier.citationVijayavenkataraman, Sanjairaj, Kannan, Sathya, Cao, Tong, Fuh, Jerry YH, Sriram, Gopu, Lu, Wen Feng (2019-10-16). 3D-Printed PCL/PPy Conductive Scaffolds as Three-Dimensional Porous Nerve Guide Conduits (NGCs) for Peripheral Nerve Injury Repair. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 7. ScholarBank@NUS Repository. https://doi.org/10.3389/fbioe.2019.00266
dc.identifier.issn22964185
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/205955
dc.description.abstractConductivity is a desirable property of an ideal nerve guide conduit (NGC) that is being considered for peripheral nerve regeneration. Most of the conductive polymers reported in use for fabrication of tissue engineering scaffolds such as polypyrrole (PPy), polyaniline, polythiophene, and poly(3,4-ethylenedioxythiophene) are non-biodegradable and possess weak mechanical properties to be fabricated into 3D structures. In this study, a biodegradable and conductive block copolymer of PPy and Polycaprolactone (PPy-b-PCL) was used to fabricate 3D porous NGCs using a novel electrohydrodynamic jet 3D printing process which offers superior control over fiber diameter, pore size, porosity, and fiber alignment. PCL/PPy scaffolds with three different concentrations of PPy-b-PCL (0.5, 1, and 2% v/v) were fabricated as a mesh (pore size 125 ± 15 μm) and the effect of incorporation of PPy-b-PCL on mechanical properties, biodegradability, and conductivity of the NGCs were studied. The mechanical properties of the scaffolds decreased with the addition of PPy-b-PCL which aided the ability to fabricate softer scaffolds that are closer to the properties of the native human peripheral nerve. With increasing concentrations of PPy-b-PCL, the scaffolds displayed a marked increase in conductivity (ranging from 0.28 to 1.15 mS/cm depending on concentration of PPy). Human embryonic stem cell-derived neural crest stem cells (hESC-NCSCs) were used to investigate the impact of PPy-b-PCL based conductive scaffolds on the growth and differentiation to peripheral neuronal cells. The hESC-NCSCs were able to attach and differentiate to peripheral neurons on PCL and PCL/PPy scaffolds, in particular the PCL/PPy (1% v/v) scaffolds supported higher growth of neural cells and a stronger maturation of hESC-NCSCs to peripheral neuronal cells. Overall, these results suggest that PPy-based conductive scaffolds have potential clinical value as cell-free or cell-laden NGCs for peripheral neuronal regeneration.
dc.language.isoen
dc.publisherFRONTIERS MEDIA SA
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiotechnology & Applied Microbiology
dc.subjectMultidisciplinary Sciences
dc.subjectScience & Technology - Other Topics
dc.subjectEHD-jet 3D printing
dc.subjectnerve guide conduit
dc.subjecttissue engineering scaffolds
dc.subjectconductive scaffolds
dc.subjectstem cells
dc.subjectperipheral nerve injury
dc.subjectELECTRICAL-STIMULATION
dc.subjectNANOFIBROUS SCAFFOLDS
dc.subjectPOLYPYRROLE
dc.subjectELECTROSPUN
dc.subjectPOLYMERS
dc.subjectDESIGN
dc.subjectSENSOR
dc.subjectRAMAN
dc.typeArticle
dc.date.updated2021-11-09T21:46:32Z
dc.contributor.departmentDEPT OF MECHANICAL ENGINEERING
dc.contributor.departmentFAC OF DENTISTRY
dc.description.doi10.3389/fbioe.2019.00266
dc.description.sourcetitleFRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
dc.description.volume7
dc.published.statePublished
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