Please use this identifier to cite or link to this item: https://doi.org/10.1002/adbi.202000146
Title: Cell-Derived Vesicles as TRPC1 Channel Delivery Systems for the Recovery of Cellular Respiratory and Proliferative Capacities
Authors: Kurth, Felix
Tai, Yee Kit 
Parate, Dinesh 
van Oostrum, Marc
Schmid, Yannick RF
Toh, Shi Jie 
Yap, Jasmine Lye Yee 
Wollscheid, Bernd
Othman, Alaa
Dittrich, Petra S
Franco-Obregon, Alfredo 
Keywords: Science & Technology
Technology
Materials Science, Biomaterials
Materials Science
cell-derived vesicles
CRISPR
Cas9 genome editing
mitochondrial respiration
pulsed magnetic fields
TRPC1 cation channel
SKELETAL-MUSCLE
MITOCHONDRIAL DYSFUNCTION
C2C12 MYOBLASTS
CA2+ ENTRY
LONGEVITY
EXERCISE
PATHWAY
SERUM
STIM1
COLD
Issue Date: 2-Sep-2020
Publisher: WILEY-V C H VERLAG GMBH
Citation: Kurth, Felix, Tai, Yee Kit, Parate, Dinesh, van Oostrum, Marc, Schmid, Yannick RF, Toh, Shi Jie, Yap, Jasmine Lye Yee, Wollscheid, Bernd, Othman, Alaa, Dittrich, Petra S, Franco-Obregon, Alfredo (2020-09-02). Cell-Derived Vesicles as TRPC1 Channel Delivery Systems for the Recovery of Cellular Respiratory and Proliferative Capacities. ADVANCED BIOSYSTEMS 4 (11). ScholarBank@NUS Repository. https://doi.org/10.1002/adbi.202000146
Abstract: Pulsed electromagnetic fields (PEMFs) are capable of specifically activating a TRPC1-mitochondrial axis underlying cell expansion and mitohormetic survival adaptations. This study characterizes cell-derived vesicles (CDVs) generated from C2C12 murine myoblasts and shows that they are equipped with the sufficient molecular machinery to confer mitochondrial respiratory capacity and associated proliferative responses upon their fusion with recipient cells. CDVs derived from wild type C2C12 myoblasts include the cation-permeable transient receptor potential (TRP) channels, TRPC1 and TRPA1, and directly respond to PEMF exposure with TRPC1-mediated calcium entry. By contrast, CDVs derived from C2C12 muscle cells in which TRPC1 has been genetically knocked-down using CRISPR/Cas9 genome editing, do not. Wild type C2C12-derived CDVs are also capable of restoring PEMF-induced proliferative and mitochondrial activation in two C2C12-derived TRPC1 knockdown clonal cell lines in accordance to their endogenous degree of TRPC1 suppression. C2C12 wild type CDVs respond to menthol with calcium entry and accumulation, likewise verifying TRPA1 functional gating and further corroborating compartmental integrity. Proteomic and lipidomic analyses confirm the surface membrane origin of the CDVs providing an initial indication of the minimal cellular machinery required to recover mitochondrial function. CDVs hence possess the potential of restoring respiratory and proliferative capacities to senescent cells and tissues.
Source Title: ADVANCED BIOSYSTEMS
URI: https://scholarbank.nus.edu.sg/handle/10635/205784
ISSN: 23667478
DOI: 10.1002/adbi.202000146
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