Please use this identifier to cite or link to this item: https://doi.org/10.1093/jb/mvz088
Title: Aurora kinase A-mediated phosphorylation of mPOU at a specific site drives skeletal muscle differentiation
Authors: Karthigeyan, Dhanasekan
Bose, Arnab
Boopathi, Ramachandran
Rao, Vinay Jaya
Shima, Hiroki
Bharathy, Narendra
Igarashi, Kazuhiko
RESHMA TANEJA 
Trivedi, Arun Kumar
Kundu, Tapas K
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Aurora kinase A
DNA binding
mitosis
mPOU
skeletal muscle differentiation
POU DOMAIN
TRANSCRIPTION FACTOR
BINDING
MYOGENESIS
FAMILY
GENE
Issue Date: 1-Feb-2020
Publisher: Oxford University Press
Citation: Karthigeyan, Dhanasekan, Bose, Arnab, Boopathi, Ramachandran, Rao, Vinay Jaya, Shima, Hiroki, Bharathy, Narendra, Igarashi, Kazuhiko, RESHMA TANEJA, Trivedi, Arun Kumar, Kundu, Tapas K (2020-02-01). Aurora kinase A-mediated phosphorylation of mPOU at a specific site drives skeletal muscle differentiation. JOURNAL OF BIOCHEMISTRY 167 (2) : 195-201. ScholarBank@NUS Repository. https://doi.org/10.1093/jb/mvz088
Abstract: Aurora kinases are Ser/Thr-directed protein kinases which play pivotal roles in mitosis. Recent evidences highlight the importance of these kinases in multiple biological events including skeletal muscle differentiation. Our earlier study identified the transcription factor POU6F1 (or mPOU) as a novel Aurora kinase (Aurk) A substrate. Here, we report that Aurora kinase A phosphorylates mPOU at Ser197 and inhibit its DNA-binding ability. Delving into mPOU physiology, we find that the phospho-mimic (S197D) mPOU mutant exhibits enhancement, while the wild type or the phospho-deficient mutant shows retardation in C2C12 myoblast differentiation. Interestingly, POU6F1 depletion phenocopies S197D-mPOU overexpression in the differentiation context. Collectively, our results signify mPOU as a negative regulator of skeletal muscle differentiation and strengthen the importance of AurkA in skeletal myogenesis.
Source Title: JOURNAL OF BIOCHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/200313
ISSN: 0021-924X
1756-2651
DOI: 10.1093/jb/mvz088
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