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Title: Agrobacterium VirE3 Uses Its Two Tandem Domains at the C-Terminus to Retain Its Companion VirE2 on the Cytoplasmic Side of the Host Plasma Membrane
Authors: Li, X. 
Zhu, T. 
Tu, H.
Pan, S.Q. 
Keywords: Agrobacterium
plasma membrane
Issue Date: 2020
Publisher: Frontiers Media S.A.
Citation: Li, X., Zhu, T., Tu, H., Pan, S.Q. (2020). Agrobacterium VirE3 Uses Its Two Tandem Domains at the C-Terminus to Retain Its Companion VirE2 on the Cytoplasmic Side of the Host Plasma Membrane. Frontiers in Plant Science 11 : 464. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Agrobacterium tumefaciens is the causal agent of crown gall disease in nature; in the laboratory the bacterium is widely used for plant genetic modification. The bacterium delivers a single-stranded transferred DNA (T-DNA) and a group of crucial virulence proteins into host cells. A putative T-complex is formed inside host cells that is composed of T-DNA and virulence proteins VirD2 and VirE2, which protect the foreign DNA from degradation and guide its way into the host nucleus. However, little is known about how the T-complex is assembled inside host cells. We combined the split-GFP and split-sfCherry labeling systems to study the interaction of Agrobacterium-delivered VirE2 and VirE3 in host cells. Our results indicated that VirE2 co-localized with VirE3 on the cytoplasmic side of the host cellular membrane upon the delivery. We identified and characterized two tandem domains at the VirE3 C-terminus that interacted with VirE2 in vitro. Deletion of these two domains abolished the VirE2 accumulation on the host plasma membrane and affected the transformation. Furthermore, the two VirE2-interacting domains of VirE3 exhibited different affinities with VirE2. Collectively, this study demonstrates that the anchorage protein VirE3 uses the two tandem VirE2-interacting domains to facilitate VirE2 protection for T-DNA at the cytoplasmic side of the host cell entrance. @ Copyright @ 2020 Li, Zhu, Tu and Pan.
Source Title: Frontiers in Plant Science
ISSN: 1664-462X
DOI: 10.3389/fpls.2020.00464
Rights: Attribution 4.0 International
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