Please use this identifier to cite or link to this item: https://doi.org/10.1126/sciadv.abb7944
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dc.titleMassively parallel single-molecule telomere length measurement with digital real-time PCR
dc.contributor.authorLuo, Y.
dc.contributor.authorViswanathan, R.
dc.contributor.authorHande, M.P.
dc.contributor.authorLoh, A.H.P.
dc.contributor.authorCheow, L.F.
dc.date.accessioned2021-08-23T03:25:04Z
dc.date.available2021-08-23T03:25:04Z
dc.date.issued2020-08-21
dc.identifier.citationLuo, Y., Viswanathan, R., Hande, M.P., Loh, A.H.P., Cheow, L.F. (2020-08-21). Massively parallel single-molecule telomere length measurement with digital real-time PCR. Science Advances 6 (34) : eabb7944. ScholarBank@NUS Repository. https://doi.org/10.1126/sciadv.abb7944
dc.identifier.issn23752548
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/198767
dc.description.abstractTelomere length is a promising biomarker for age-associated diseases and cancer, but there are still substantial challenges to routine telomere analysis in clinics because of the lack of a simple and rapid yet scalable method for measurement. We developed the single telomere absolute-length rapid (STAR) assay, a novel high-throughput digital real-time PCR approach for rapidly measuring the absolute lengths and quantities of individual telomere molecules. We show that this technique provides the accuracy and sensitivity to uncover associations between telomere length distribution and telomere maintenance mechanisms in cancer cell lines and primary tumors. The results indicate that the STAR assay is a powerful tool to enable the use of telomere length distribution as a biomarker in disease and population-wide studies. Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
dc.publisherAmerican Association for the Advancement of Science
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.sourceScopus OA2020
dc.typeArticle
dc.contributor.departmentINST FOR HEALTH INNOVATION & TECHNOLOGY
dc.contributor.departmentDEPT OF PHYSIOLOGY
dc.contributor.departmentDEPT OF BIOMEDICAL ENGINEERING
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1126/sciadv.abb7944
dc.description.sourcetitleScience Advances
dc.description.volume6
dc.description.issue34
dc.description.pageeabb7944
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