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Title: Megavoltage radiosensitization of gold nanoparticles on a glioblastoma cancer cell line using a clinical platform
Authors: Kazmi, F.
Vallis, K.A.
Vellayappan, B.A.
Bandla, A. 
Yukun, D. 
Carlisle, R.
Keywords: External beam radiotherapy
Glioblastoma multiform
Issue Date: 2020
Publisher: MDPI AG
Citation: Kazmi, F., Vallis, K.A., Vellayappan, B.A., Bandla, A., Yukun, D., Carlisle, R. (2020). Megavoltage radiosensitization of gold nanoparticles on a glioblastoma cancer cell line using a clinical platform. International Journal of Molecular Sciences 21 (2) : 429. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Gold nanoparticles (GNPs) have demonstrated significant dose enhancement with kilovoltage (kV) X-rays; however, recent studies have shown inconsistent findings with megavoltage (MV) X-rays. We propose to evaluate the radiosensitization effect on U87 glioblastoma (GBM) cells in the presence of 42 nm GNPs and irradiated with a clinical 6 MV photon beam. Cytotoxicity and radiosensitization were measured using MTS and clonogenic cellular radiation sensitivity assays, respectively. The sensitization enhancement ratio was calculated for 2 Gy (SER2Gy) with GNP (100 µg/mL). Dark field and MTS assays revealed high co-localization and good biocompatibility of the GNPs with GBM cells. A significant sensitization enhancement of 1.45 (p = 0.001) was observed with GNP 100 µg/mL. Similarly, at 6 Gy, there was significant difference in the survival fraction between the GBM alone group (mean (M) = 0.26, standard deviation (SD) = 0.008) and the GBM plus GNP group (M = 0.07, SD = 0.05, p = 0.03). GNPs enabled radiosensitization in U87 GBM cells at 2 Gy when irradiated using a clinical platform. In addition to the potential clinical utility of GNPs, these studies demonstrate the effectiveness of a robust and easy to standardize an in-vitro model that can be employed for future studies involving metal nanoparticle plus irradiation. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: International Journal of Molecular Sciences
ISSN: 1661-6596
DOI: 10.3390/ijms21020429
Rights: Attribution 4.0 International
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