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https://doi.org/10.3390/v12020195
Title: | A conserved carboxylesterase inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana plants | Authors: | Guo, S. Wong, Sek-Man |
Keywords: | Carboxylesterase Coat protein Host resistance Tobacco mosaic virus |
Issue Date: | 2020 | Publisher: | MDPI AG | Citation: | Guo, S., Wong, Sek-Man (2020). A conserved carboxylesterase inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana plants. Viruses 12 (2) : 195. ScholarBank@NUS Repository. https://doi.org/10.3390/v12020195 | Rights: | Attribution 4.0 International | Abstract: | A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate. In plants, CXEs have been implicated in defense, development, and secondary metabolism. We discovered a new CXE gene in Nicotiana benthamiana that is related to virus resistance. The transcriptional level of NbCXE expression was significantly increased after Tobacco mosaic virus (TMV) infection. Transient over-expression of NbCXE inhibited TMV accumulation in N. benthamiana plants. Conversely, when the NbCXE gene was silenced with a Tobacco rattle virus (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection. NbCXE protein was shown to interact with TMV coat protein (CP) in vitro. Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced N. benthamiana plants. In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | Source Title: | Viruses | URI: | https://scholarbank.nus.edu.sg/handle/10635/197873 | ISSN: | 19994915 | DOI: | 10.3390/v12020195 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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