Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.ymthe.2021.05.008
DC Field | Value | |
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dc.title | Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer. | |
dc.contributor.author | Tai Tay, Daryl Jin | |
dc.contributor.author | Song, Yangyang | |
dc.contributor.author | Peng, Boya | |
dc.contributor.author | Toh, Tan Boon | |
dc.contributor.author | Hooi, Lissa | |
dc.contributor.author | Kaixin Toh, Desiree-Faye | |
dc.contributor.author | Hong, HuiQi | |
dc.contributor.author | Tang, Sze Jing | |
dc.contributor.author | Han, Jian | |
dc.contributor.author | Gan, Wei Liang | |
dc.contributor.author | Man Chan, Tim Hon | |
dc.contributor.author | Krishna, Manchugondanahalli S | |
dc.contributor.author | Patil, Kiran M | |
dc.contributor.author | Maraswami, Manikantha | |
dc.contributor.author | Loh, Teck Peng | |
dc.contributor.author | Dan, Yock Young | |
dc.contributor.author | Zhou, Lei | |
dc.contributor.author | Bonney, Glenn Kunnath | |
dc.contributor.author | Kah-Hoe Chow, Pierce | |
dc.contributor.author | Chen, Gang | |
dc.contributor.author | Kai-Hua Chow, Edward | |
dc.contributor.author | Le, Minh Tn | |
dc.contributor.author | Chen, Leilei | |
dc.date.accessioned | 2021-06-23T07:53:32Z | |
dc.date.available | 2021-06-23T07:53:32Z | |
dc.date.issued | 2021-05-08 | |
dc.identifier.citation | Tai Tay, Daryl Jin, Song, Yangyang, Peng, Boya, Toh, Tan Boon, Hooi, Lissa, Kaixin Toh, Desiree-Faye, Hong, HuiQi, Tang, Sze Jing, Han, Jian, Gan, Wei Liang, Man Chan, Tim Hon, Krishna, Manchugondanahalli S, Patil, Kiran M, Maraswami, Manikantha, Loh, Teck Peng, Dan, Yock Young, Zhou, Lei, Bonney, Glenn Kunnath, Kah-Hoe Chow, Pierce, Chen, Gang, Kai-Hua Chow, Edward, Le, Minh Tn, Chen, Leilei (2021-05-08). Targeting RNA Editing of Antizyme Inhibitor 1: a Potential Oligonucleotide-Based Antisense Therapy for Cancer.. Mol Ther. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ymthe.2021.05.008 | |
dc.identifier.issn | 15250016 | |
dc.identifier.issn | 15250024 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/192146 | |
dc.description.abstract | Dysregulated adenosine-to-inosine (A-to-I) RNA editing is implicated in various cancers. However, no available RNA editing inhibitors have so far been developed to inhibit cancer-associated RNA editing events. Here we decipher the RNA secondary structure of antizyme inhibitor 1 (AZIN1), one of the best-studied A-to-I editing targets in cancer, by locating its editing site complementary sequence (ECS) at the 3'end of exon 12. Chemically modified antisense oligonucleotides (ASOs) which target the editing region of AZIN1 caused a substantial exon 11 skipping; while ECS-targeting ASOs effectively abolish AZIN1 editing without affecting splicing and translation. We demonstrate that complete 2'-O-methyl (2'-O-Me) sugar ring modification in combination with partial phosphorothioate (PS) backbone modification may be an optimal chemistry for editing inhibition. ASO3.2, which targets the ECS, specifically inhibits cancer cell viability in vitro and tumor incidence and growth in xenograft models. Our results demonstrate that this AZIN1-targeting, ASO-based therapeutics may be applicable to a wide range of tumor types. | |
dc.publisher | Elsevier BV | |
dc.source | Elements | |
dc.subject | A-to-I RNA editing | |
dc.subject | ADAR1 | |
dc.subject | AZIN1 | |
dc.subject | Cancer | |
dc.subject | RNA therapeutics | |
dc.subject | antisense oligonucleotides | |
dc.type | Article | |
dc.date.updated | 2021-06-23T05:41:00Z | |
dc.contributor.department | BIOMED INST FOR GLOBAL HEALTH RES & TECH | |
dc.contributor.department | CANCER SCIENCE INSTITUTE OF SINGAPORE | |
dc.contributor.department | DEPT OF ANATOMY | |
dc.contributor.department | DEPT OF MEDICINE | |
dc.contributor.department | DEPT OF PHARMACOLOGY | |
dc.contributor.department | DUKE-NUS MEDICAL SCHOOL | |
dc.contributor.department | LIFE SCIENCES INSTITUTE | |
dc.description.doi | 10.1016/j.ymthe.2021.05.008 | |
dc.description.sourcetitle | Mol Ther | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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File | Description | Size | Format | Access Settings | Version | |
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Targeting RNA Editing of Antizyme Inhibitor 1- a Potential Oligonucleotide-Based Antisense Therapy for Cancer.pdf | Accepted version | 6.43 MB | Adobe PDF | CLOSED | None |
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