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Title: Quantitative Phase Imaging and Reconstruction For Biological Applications
Keywords: biomedical optics, quantitative phase imaging, optical microscopy
Issue Date: 7-May-2010
Citation: KOU SHAN SHAN (2010-05-07). Quantitative Phase Imaging and Reconstruction For Biological Applications. ScholarBank@NUS Repository.
Abstract: The technology of quantitative phase microscopy is not widely applied in today's biological and biomedical research laboratories, because in general, researchers do not understand what sort of information phase imaging is providing and how to interpret it from genotype or relate it to phenotype. This dissertation is dedicated to the methodologies that derive optical phase information from microscopic instrumentation and imaging techniques. A few such latest and signi cant techniques are thoroughly studied through both theoretical formalism as well as experimental veri cations. Some interesting and novel algorithms that not only enhance these technologies but also interlink and open up new regimes for achieving quantitative phase imaging are proposed and results presented. Despite the di culties in manipulating real biological samples, many of the experiments and applications presented here are based on such specimens, and sometimes, even live. It is the hope that this thesis will exploit the opportunities that phase imaging and three-dimensional reconstructions can present for biological and biomedical applications through the framework of both theoretical analysis and instrumental reconstructions.
Appears in Collections:Ph.D Theses (Open)

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