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Title: Establishment of Autologous Culture Systems for Human Embryonic Stem Cells
Authors: FU XIN
Keywords: human embryonic stem cells, cell culture, autologous, feeder-free
Issue Date: 11-Aug-2010
Citation: FU XIN (2010-08-11). Establishment of Autologous Culture Systems for Human Embryonic Stem Cells. ScholarBank@NUS Repository.
Abstract: Human embryonic stem cells (hESCs) hold great promise for regenerative medicine due to their unlimited differentiation potential and proliferation capacity. Currently, potential clinical and experimental applications of hESCs are hampered by the use of mouse embryonic fibroblasts as feeder layer and animal-derived components during culture. In this study, autologous fibroblasts derived from H9 hESCs with initial embryoid body formation (H9 ebF) could serve as an optimal feeder system to support prolonged undifferentiated culture of hESCs. The extracellular matrix extracted from H9 ebF can further support feeder-free growth H1 and H9 hESC lines in the animal component free and serum-free TeSR-2 medium. This study suggests a possible direction for the future improvement in hESC culture system. This study also advances the development of clinical-grade hESCs for both pre-clinical and clinical applications in future.
Appears in Collections:Ph.D Theses (Open)

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