Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/187663
DC FieldValue
dc.titleMEDICINAL PLANTS AND CYCLIC NUCLEOTIDE PHOSPHODIESTERASE INHIBITORY ACTIVITY
dc.contributor.authorSIN JIA EN VALERIE
dc.date.accessioned2021-03-26T18:00:22Z
dc.date.available2021-03-26T18:00:22Z
dc.date.issued2020-11-30
dc.identifier.citationSIN JIA EN VALERIE (2020-11-30). MEDICINAL PLANTS AND CYCLIC NUCLEOTIDE PHOSPHODIESTERASE INHIBITORY ACTIVITY. ScholarBank@NUS Repository.
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/187663
dc.description.abstractErectile dysfunction is a distressing condition afflicting men of all ages but current treatment options face limitations. Hence, this work aims to investigate the PDE5 inhibitory potential of Swietenia macrophylla and Vitex trifolia, evaluate the in vitro cytotoxicity of S. macrophylla and develop a novel PDE5 inhibition screening method. S. macrophylla possessed more potent PDE5 inhibition than V. trifolia. Subsequent bioassay-guided fractionation of the most active S. macrophylla ethyl acetate maceration extract revealed that subfraction EM5-3 is a promising source for PDE5 inhibitors. In vitro cytotoxicity screening of its extracts, column fractions and selected phytoconstituents demonstrated that only non-polar extracts and fatty acids significantly reduced L02 and Vero cell viabilities at high concentrations. A novel fluorescence polarization-based PDE5 inhibitory screening assay was successfully developed and applied to selected S. macrophylla extracts, fractions, compounds, sildenafil and its analogues. Overall, the findings of this work provide scientific evidence to support the reported use of S. macrophylla in sexual performance enhancement and it represents a step towards the development of novel and effective PDE5 inhibitors
dc.language.isoen
dc.subjectMedicinal plants, phosphodiesterase 5, erectile dysfunction, bioassay guided fractionation, cytotoxicity, fluorescence polarisation
dc.typeThesis
dc.contributor.departmentPHARMACY
dc.contributor.supervisorHwee Ling Koh
dc.contributor.supervisorGanesh Srinivasan Anand
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY (FOS)
Appears in Collections:Ph.D Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
SinJEV.pdf4.33 MBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.