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Title: | ROLE OF FREE AND CO-RECEPTOR BOUND LCK IN THYMOCYTES AND PERIPHERAL CD8+ T CELLS | Authors: | WEI QIANRU | Keywords: | T cell signaling, Lck, coreceptor, CD8, OT-I hybridoma | Issue Date: | 21-Aug-2018 | Citation: | WEI QIANRU (2018-08-21). ROLE OF FREE AND CO-RECEPTOR BOUND LCK IN THYMOCYTES AND PERIPHERAL CD8+ T CELLS. ScholarBank@NUS Repository. | Abstract: | Lck is critical for T cell activation, as it phosphorylates the TCR/CD3 complex. Lck associates with CD4 and CD8 co-receptors, but it can also exist in a coreceptor-unbound (free) form. We compared the phosphorylation states of coreceptor-bound and free Lck. The free Lck pool has a higher degree of Y394 phosphorylation than coreceptor-bound Lck pool. We also investigated the mobility of free and coreceptor-bound Lck by FRAP imaging. Free Lck showed higher mobility compared to CD8α-bound Lck. Finally, we quantified the percentage of coreceptors that bound to Lck by Flow Cytometry Immunoprecipitation (FC-IP). The coreceptor and Lck coupling increases during the thymocyte development, but decreases upon TCR activation in the periphery. This enhanced activity and mobility of free Lck could enable free Lck to initiate TCR signaling more rapidly than coreceptor-bound Lck. The coreceptor and Lck coupling changes during the thymocyte development and TCR activation indicate functional differences between these two pools of Lck. | URI: | https://scholarbank.nus.edu.sg/handle/10635/185997 |
Appears in Collections: | Ph.D Theses (Open) |
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Thesis-Qianru (Final electronic submission).pdf | 3.03 MB | Adobe PDF | OPEN | None | View/Download |
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