Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/185275
Title: VIRUS-HOST INTERACTIONS OF THE MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS (MERS-COV) NUCLEOCAPSID PROTEIN
Authors: JAMES ODAME ABOAGYE
Keywords: MERS-CoV, MERS-N, virus-host, interactions, antiviral, CXCL10
Issue Date: 17-Aug-2018
Citation: JAMES ODAME ABOAGYE (2018-08-17). VIRUS-HOST INTERACTIONS OF THE MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS (MERS-COV) NUCLEOCAPSID PROTEIN. ScholarBank@NUS Repository.
Abstract: The novel human coronavirus MERS-CoV causes severe respiratory distress with a high mortality rate. The nucleocapsid (N) protein of MERS-CoV (MERS-N) interacts with host factors to regulate cellular functions. To determine the processing and subcellular localization of MERS-N as well as its role in regulating host functions, mouse monoclonal antibodies binding to different domains within MERS-N were generated to detect its expression in infected and overexpressed cells. Employing the monoclonal antibodies, the N protein was cleaved in transfected and infected cells with additional cleavage/degradation and/or modification(s) observed in infected cells. PCR array was used to screen for the regulation of host antiviral response and apoptotic genes. Several genes were upregulated transcriptionally including IL6, IL8, TNF, CXCL10, and BCL2. CXCL10 was upregulated on the translational level via the C-terminal fragment (196-413) of MERS-N. In addition, MERS-N was characterized as a nucleocytoplasmic protein with functional nuclear export and nuclear localization signals. The localization of MERS-N within the cytoplasm contributes to the upregulation of CXCL10. Also, the N protein was observed to interact with chromatin. This study shed light on the possible role of the N protein in the replication and pathogenesis of MERS-CoV.
URI: https://scholarbank.nus.edu.sg/handle/10635/185275
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