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Title: Structural Insights into Plant Cell Proliferation Disturbance by Agrobacterium Protein 6B
Keywords: Agrobacterium, RolB family, ADP-ribosylation, miRNA
Issue Date: 26-Oct-2010
Citation: WANG MEIMEI (2010-10-26). Structural Insights into Plant Cell Proliferation Disturbance by Agrobacterium Protein 6B. ScholarBank@NUS Repository.
Abstract: The Agrobacterium T-DNA 6b protein has weak sequence similarity to other RolB family proteins, which can cause callus formation on tobacco leaf discs without the presence of auxin. 6b proteins interact with many different proteins involved in expression of plant genes related to cell proliferation. Here, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 Å and Agrobacterium vitis AB6b at 1.65 Å. The 6b structure adopts an ADP-ribosylating toxin fold and represents a new toxin family with Tyr66, Thr93 and Tyr153 as the putative ADP-ribosylation catalytic residues. We showed that overexpression of 6b in Arabidopsis displays miRNA deficiency phenotype and a specific loop fragment (residues 40-55) of 6b is needed for specific direct binding to SERRATE and AGO1. Our work provides structural insights suggesting that 6b affects the growth of plant cells probably via its ADP-ribosylation activity and the disturbance of miRNA pathway.
Appears in Collections:Master's Theses (Open)

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