Please use this identifier to cite or link to this item: https://doi.org/10.1021/bi401270d
Title: Targeting RNA-protein interactions within the human immunodeficiency virus type 1 lifecycle
Authors: Bell, N.M
L'Hernault, A
Murat, P
Richards, J.E
Lever, A.M.L 
Balasubramanian, S
Keywords: Diseases
Genes
Life cycle
Molecules
Proteins
Viruses
H NMR spectroscopy
Human immunodeficiency virus type-1
Inhibitory activity
RNA-protein interactions
Structural elements
Structural proteins
Therapeutic targets
Viral replication
RNA
Gag protein
structural protein
virus protein
virus RNA
anti human immunodeficiency virus agent
molecular library
NSC-260594
quinoline derivative
ribonucleoprotein
Schiff base
spliced leader RNA
virus RNA
anti human immunodeficiency virus agent
Gag protein
NSC 260594
ribonucleoprotein
spliced leader RNA
5' untranslated region
article
controlled study
human
human cell
Human immunodeficiency virus 1
in vitro study
life cycle stage
molecule
nonhuman
priority journal
protein expression
protein RNA binding
proton nuclear magnetic resonance
virus genome
virus infectivity
virus particle
virus replication
antagonists and inhibitors
binding site
cell survival
chemical structure
chemistry
conformation
DNA denaturation
drug effects
drug screening
HEK293 cell line
HeLa cell line
HIV Infections
Human immunodeficiency virus 1
metabolism
molecular library
molecularly targeted therapy
osmolarity
physiology
cell strain HEK293
drug antagonism
drug effect
HeLa cell
Human immunodeficiency virus infection
virus replication
Anti-HIV Agents
Binding Sites
Cell Survival
Drug Evaluation, Preclinical
gag Gene Products, Human Immunodeficiency Virus
HEK293 Cells
HeLa Cells
HIV Infections
HIV-1
Humans
Models, Molecular
Molecular Targeted Therapy
Nucleic Acid Conformation
Nucleic Acid Denaturation
Osmolar Concentration
Quinolinium Compounds
Ribonucleoproteins
RNA, Spliced Leader
RNA, Viral
Schiff Bases
Small Molecule Libraries
Virus Replication
Anti-HIV Agents
Binding Sites
Cell Survival
Drug Evaluation, Preclinical
gag Gene Products, Human Immunodeficiency Virus
HEK293 Cells
HeLa Cells
HIV Infections
HIV-1
Humans
Models, Molecular
Molecular Targeted Therapy
Nucleic Acid Conformation
Nucleic Acid Denaturation
Osmolar Concentration
Quinolinium Compounds
Ribonucleoproteins
RNA, Spliced Leader
RNA, Viral
Schiff Bases
Small Molecule Libraries
Virus Replication
Issue Date: 2013
Publisher: American Chemical Society
Citation: Bell, N.M, L'Hernault, A, Murat, P, Richards, J.E, Lever, A.M.L, Balasubramanian, S (2013). Targeting RNA-protein interactions within the human immunodeficiency virus type 1 lifecycle. Biochemistry 52 (51) : 9269-9274. ScholarBank@NUS Repository. https://doi.org/10.1021/bi401270d
Rights: Attribution 4.0 International
Abstract: RNA-protein interactions are vital throughout the HIV-1 life cycle for the successful production of infectious virus particles. One such essential RNA-protein interaction occurs between the full-length genomic viral RNA and the major structural protein of the virus. The initial interaction is between the Gag polyprotein and the viral RNA packaging signal (psi or ?), a highly conserved RNA structural element within the 5?-UTR of the HIV-1 genome, which has gained attention as a potential therapeutic target. Here, we report the application of a target-based assay to identify small molecules, which modulate the interaction between Gag and ?. We then demonstrate that one such molecule exhibits potent inhibitory activity in a viral replication assay. The mode of binding of the lead molecules to the RNA target was characterized by 1H NMR spectroscopy. © 2013 American Chemical Society.
Source Title: Biochemistry
URI: https://scholarbank.nus.edu.sg/handle/10635/183903
ISSN: 0006-2960
DOI: 10.1021/bi401270d
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications
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