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Title: Establishing criteria for human mesenchymal stem cell potency
Authors: Samsonraj, R.M
Rai, B
Sathiyanathan, P
Puan, K.J
Rötzschke, O
Hui, J.H 
Raghunath, M
Stanton, L.W 
Nurcombe, V
Cool, S.M 
Keywords: biological marker
cell surface marker
DERMO 1 protein
growth factor
messenger RNA
peptides and proteins
platelet derived growth factor alpha receptor
STRO 1 protein
TWIST 1 protein
unclassified drug
bone marrow
cell differentiation
cell growth
cell isolation
cell size
colony formation
controlled study
genetic transcription
human cell
in vitro study
in vivo study
mesenchymal stem cell
protein expression
tissue regeneration
bone marrow cell
cell culture
cell proliferation
mesenchymal stroma cell
wound healing
Bone Marrow Cells
Cell Differentiation
Cell Proliferation
Cells, Cultured
Mesenchymal Stromal Cells
Wound Healing
Issue Date: 2015
Publisher: AlphaMed Press
Citation: Samsonraj, R.M, Rai, B, Sathiyanathan, P, Puan, K.J, Rötzschke, O, Hui, J.H, Raghunath, M, Stanton, L.W, Nurcombe, V, Cool, S.M (2015). Establishing criteria for human mesenchymal stem cell potency. Stem Cells 33 (6) : 1878-1891. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: This study sought to identify critical determinants of mesenchymal stem cell (MSC) potency using in vitro and in vivo attributes of cells isolated from the bone marrow of age- and sex-matched donors. Adherence to plastic was not indicative of potency, yet capacity for long-term expansion in vitro varied considerably between donors, allowing the grouping of MSCs from the donors into either those with high-growth capacity or low-growth capacity. Using this grouping strategy, high-growth capacity MSCs were smaller in size, had greater colony-forming efficiency, and had longer telomeres. Cell-surface biomarker analysis revealed that the International Society for Cellular Therapy (ISCT) criteria did not distinguish between high-growth capacity and low-growth capacity MSCs, whereas STRO-1 and platelet-derived growth factor receptor alpha were preferentially expressed on high-growth capacity MSCs. These cells also had the highest mean expression of the mRNA transcripts TWIST-1 and DERMO-1. Irrespective of these differences, both groups of donor MSCs produced similar levels of key growth factors and cytokines involved in tissue regeneration and were capable of multilineage differentiation. However, high-growth capacity MSCs produced approximately double the volume of mineralized tissue compared to low-growth capacity MSCs when assessed for ectopic bone-forming ability. The additional phenotypic criteria presented in this study when combined with the existing ISCT minimum criteria and working proposal will permit an improved assessment of MSC potency and provide a basis for establishing the quality of MSCs prior to their therapeutic application. © 2015 AlphaMed Press.
Source Title: Stem Cells
ISSN: 1066-5099
DOI: 10.1002/stem.1982
Rights: Attribution 4.0 International
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