Please use this identifier to cite or link to this item: https://doi.org/10.3390/jcm9010063
Title: Kinetics of Tear Fluid Proteins after Endothelial Keratoplasty and Predictive Factors for Recovery from Corneal Haze
Authors: Yawata, Nobuyo 
Awate, Sunita
Liu, Yu-Chi 
Yuan, Shi
Woon, Kaing 
Siak, Jay 
Kawano, Yoh-Ichi
Sonoda, Koh-Hei
Mehta, Jodhbir S 
Yawata, Makoto 
Keywords: Science & Technology
Life Sciences & Biomedicine
Medicine, General & Internal
General & Internal Medicine
endothelial keratoplasty
tear fluid cytokines
corneal haze
GENE-EXPRESSION
CYTOKINE LEVELS
GRAFT-SURVIVAL
IN-VIVO
KERATITIS
STROMA
INTERLEUKIN-1
MECHANISMS
REJECTION
NERVES
Issue Date: 1-Jan-2020
Publisher: MDPI
Citation: Yawata, Nobuyo, Awate, Sunita, Liu, Yu-Chi, Yuan, Shi, Woon, Kaing, Siak, Jay, Kawano, Yoh-Ichi, Sonoda, Koh-Hei, Mehta, Jodhbir S, Yawata, Makoto (2020-01-01). Kinetics of Tear Fluid Proteins after Endothelial Keratoplasty and Predictive Factors for Recovery from Corneal Haze. JOURNAL OF CLINICAL MEDICINE 9 (1). ScholarBank@NUS Repository. https://doi.org/10.3390/jcm9010063
Abstract: Endothelial keratoplasty (EK) is less invasive with faster recovery as compared to conventional penetrating keratoplasty, however, it relies on the clarity of the host corneal stroma. Corneal transplantation involves the induction of immune tolerance for allogeneic tissues as well as the corneal wound healing process, in which coordinated interactions between cytokines and growth factors are critical. In this study, we profiled the expression of 51 soluble factors in the tear fluid over the course of EK and have provided evidence of dynamic changes in cytokine expression in the ipsilateral and contralateral eyes. Cluster analyses classified the cytokine expression kinetics into five groups. Group 1 proteins included TGF-b1, IL-1b, and innate proinflammatory cytokines, which bilaterally increased after surgery, despite the use of topical corticosteroid in the transplanted eyes. Local corticosteroids suppressed cytokines involved in adaptive immunity in the transplanted eyes but not in the contralateral eyes. We found tear protein expression at baseline and one week post-surgery to be a potential predictive biomarker of delayed recovery after EK in terms of the corneal haze and visual acuity. Furthermore, Group 1 tear proteins were most associated with persistent corneal haze pre-surgery as well as visual acuity at one month-post transplant.
Source Title: JOURNAL OF CLINICAL MEDICINE
URI: https://scholarbank.nus.edu.sg/handle/10635/183429
ISSN: 20770383
DOI: 10.3390/jcm9010063
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