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https://doi.org/10.1186/1465-9921-11-52
Title: | Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells | Authors: | Wemhöner, A Hackspiel, I Hobi, N Ravasio, A Haller, T Rüdiger, M |
Keywords: | adenosine triphosphate cell marker fluorocarbon flutec ionomycin perfluorodecalin perfluoromethylcyclohexan phorbol 13 acetate 12 myristate surfactant unclassified drug ionomycin perfluorodecalin perfluoromethylcyclohexane phorbol 13 acetate 12 myristate surfactant associated protein animal cell article cell isolation cell membrane fluidity cell membrane permeability cell structure cell viability controlled study endocytosis exocytosis lung alveolus cell type 2 male nonhuman rat vapor pressure animal cell membrane cell shape cell survival drug effect human lung alveolus cell membrane fluidity metabolism Sprague Dawley rat time tumor cell line Adenosine Triphosphate Animals Cell Line, Tumor Cell Membrane Cell Membrane Permeability Cell Shape Cell Survival Exocytosis Fluorocarbons Humans Ionomycin Male Membrane Fluidity Pneumocytes Pulmonary Surfactant-Associated Proteins Rats Rats, Sprague-Dawley Tetradecanoylphorbol Acetate Time Factors Vapor Pressure |
Issue Date: | 2010 | Citation: | Wemhöner, A, Hackspiel, I, Hobi, N, Ravasio, A, Haller, T, Rüdiger, M (2010). Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells. Respiratory Research 11 : 52. ScholarBank@NUS Repository. https://doi.org/10.1186/1465-9921-11-52 | Rights: | Attribution 4.0 International | Abstract: | Background: Perfluorocarbons (PFC) are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus, effects on alveolar type II (ATII) cells and surfactant metabolism can be expected, data, however, are controversial.Objective: The study was performed to test two hypotheses: (I) the effects of PFC on surfactant exocytosis depend on their respective vapor pressures; (II) different pathways of surfactant exocytosis are affected differently by PFC.Methods: Isolated ATII cells were exposed to two PFC with different vapor pressures and spontaneous surfactant exocytosis was measured. Furthermore, surfactant exocytosis was stimulated by either ATP, PMA or Ionomycin. The effects of PFC on cell morphology, cellular viability, endocytosis, membrane permeability and fluidity were determined.Results: The spontaneous exocytosis was reduced by PFC, however, the ATP and PMA stimulated exocytosis was slightly increased by PFC with high vapor pressure. In contrast, Ionomycin-induced exocytosis was decreased by PFC with low vapor pressure. Cellular uptake of FM 1-43 - a marker of membrane integrity - was increased. However, membrane fluidity, endocytosis and viability were not affected by PFC incubation.Conclusions: We conclude that PFC effects can be explained by modest, unspecific interactions with the plasma membrane rather than by specific interactions with intracellular targets. © 2010 Wemhöner et al; licensee BioMed Central Ltd. | Source Title: | Respiratory Research | URI: | https://scholarbank.nus.edu.sg/handle/10635/183266 | ISSN: | 14659921 | DOI: | 10.1186/1465-9921-11-52 | Rights: | Attribution 4.0 International |
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