Please use this identifier to cite or link to this item: https://doi.org/10.1186/gb-2012-13-10-R85
Title: H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions
Authors: Ku, M
Jaffe, J.D
Koche, R.P
Rheinbay, E
Endoh, M 
Koseki, H
Carr, S.A
Bernstein, B.E
Keywords: histone H2AZ
histone H3
chromatin
histone
histone H2A.F-Z
animal cell
article
chromatin structure
controlled study
enhancer region
histone acetylation
histone methylation
histone ubiquitination
human
male
mouse
nonhuman
nucleotide sequence
pluripotent stem cell
promoter region
protein function
protein localization
protein processing
protein structure
animal
chromatin
embryonic stem cell
genome
metabolism
multipotent stem cell
neural stem cell
protein processing
transcription initiation
Mammalia
Animals
Chromatin
Embryonic Stem Cells
Enhancer Elements, Genetic
Genome
Histones
Humans
Mice
Multipotent Stem Cells
Neural Stem Cells
Pluripotent Stem Cells
Promoter Regions, Genetic
Protein Processing, Post-Translational
Transcriptional Activation
Issue Date: 2012
Citation: Ku, M, Jaffe, J.D, Koche, R.P, Rheinbay, E, Endoh, M, Koseki, H, Carr, S.A, Bernstein, B.E (2012). H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions. Genome Biology 13 (10) : R85. ScholarBank@NUS Repository. https://doi.org/10.1186/gb-2012-13-10-R85
Rights: Attribution 4.0 International
Abstract: Background: The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure.Results: We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly with H3K4 methylation. Accordingly, H2A.Z is present at poised promoters with bivalent chromatin and at active promoters with H3K4 methylation, but is absent from stably repressed promoters that are specifically enriched for H3K27 trimethylation. We also characterized post-translational modification states of H2A.Z, including a novel species dually-modified by ubiquitination and acetylation that is enriched at bivalent chromatin.Conclusions: Our findings associate H2A.Z with functionally distinct genomic elements, and suggest that post-translational modifications may reconcile its contrasting locations and roles. © 2012 Ku et al.; licensee BioMed Central Ltd.
Source Title: Genome Biology
URI: https://scholarbank.nus.edu.sg/handle/10635/183225
ISSN: 1474760X
DOI: 10.1186/gb-2012-13-10-R85
Rights: Attribution 4.0 International
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