Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep25955
Title: A novel podocyte gene, semaphorin 3G, protects glomerular podocyte from lipopolysaccharide-induced inflammation
Authors: Ishibashi, R
Takemoto, M
Akimoto, Y
Ishikawa, T
He, P
Maezawa, Y
Sakamoto, K
Tsurutani, Y
Ide, S
Ide, K
Kawamura, H
Kobayashi, K
Tokuyama, H
Tryggvason, K 
Betsholtz, C
Yokote, K
Keywords: cytokine
lipopolysaccharide
semaphorin
semaphorin 3G, human
semaphorin 3G, mouse
streptozocin
albuminuria
animal
cell culture
chemically induced
complication
cytology
diabetic nephropathy
disease model
experimental diabetes mellitus
gene knockout
genetics
human
immunology
inflammation
metabolism
mouse
podocyte
signal transduction
Albuminuria
Animals
Cells, Cultured
Cytokines
Diabetes Mellitus, Experimental
Diabetic Nephropathies
Disease Models, Animal
Gene Knockout Techniques
Humans
Inflammation
Lipopolysaccharides
Mice
Podocytes
Semaphorins
Signal Transduction
Streptozocin
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Ishibashi, R, Takemoto, M, Akimoto, Y, Ishikawa, T, He, P, Maezawa, Y, Sakamoto, K, Tsurutani, Y, Ide, S, Ide, K, Kawamura, H, Kobayashi, K, Tokuyama, H, Tryggvason, K, Betsholtz, C, Yokote, K (2016). A novel podocyte gene, semaphorin 3G, protects glomerular podocyte from lipopolysaccharide-induced inflammation. Scientific Reports 6 : 25955. ScholarBank@NUS Repository. https://doi.org/10.1038/srep25955
Rights: Attribution 4.0 International
Abstract: Kidney diseases including diabetic nephropathy have become huge medical problems, although its precise mechanisms are still far from understood. In order to increase our knowledge about the patho-physiology of kidney, we have previously identified >300 kidney glomerulus-enriched transcripts through large-scale sequencing and microarray profiling of the mouse glomerular transcriptome. One of the glomerulus-specific transcripts identified was semaphorin 3G (Sema3G) which belongs to the semaphorin family. The aim of this study was to analyze both the in vivo and in vitro functions of Sema3G in the kidney. Sema3G was expressed in glomerular podocytes. Although Sema3G knockout mice did not show obvious glomerular defects, ultrastructural analyses revealed partially aberrant podocyte foot processes structures. When these mice were injected with lipopolysaccharide to induce acute inflammation or streptozotocin to induce diabetes, the lack of Sema3G resulted in increased albuminuria. The lack of Sema3G in podocytes also enhanced the expression of inflammatory cytokines including chemokine ligand 2 and interleukin 6. On the other hand, the presence of Sema3G attenuated their expression through the inhibition of lipopolysaccharide-induced Toll like receptor 4 signaling. Taken together, our results surmise that the Sema3G protein is secreted by podocytes and protects podocytes from inflammatory kidney diseases and diabetic nephropathy. © 2016, Nature Publishing Group. All rights reserved.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/182472
ISSN: 2045-2322
DOI: 10.1038/srep25955
Rights: Attribution 4.0 International
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