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Title: Engineering a 3D microfluidic culture platform for tumor-treating field application
Authors: Pavesi, A
Adriani, G
Tay, A 
Warkiani, M.E
Yeap, W.H
Wong, S.C 
Kamm, R.D
Keywords: A-549 cell line
cell proliferation
cell survival
endothelium cell
equipment design
gene expression regulation
microfluidic analysis
tumor cell line
umbilical vein endothelial cell
A549 Cells
Cell Line, Tumor
Cell Proliferation
Cell Survival
Coculture Techniques
Electric Stimulation Therapy
Endothelial Cells
Equipment Design
Gene Expression Regulation, Neoplastic
Human Umbilical Vein Endothelial Cells
Microfluidic Analytical Techniques
Issue Date: 2016
Publisher: Nature Publishing Group
Citation: Pavesi, A, Adriani, G, Tay, A, Warkiani, M.E, Yeap, W.H, Wong, S.C, Kamm, R.D (2016). Engineering a 3D microfluidic culture platform for tumor-treating field application. Scientific Reports 6 : 26584. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The limitations of current cancer therapies highlight the urgent need for a more effective therapeutic strategy. One promising approach uses an alternating electric field; however, the mechanisms involved in the disruption of the cancer cell cycle as well as the potential adverse effects on non-cancerous cells must be clarified. In this study, we present a novel microfluidic device with embedded electrodes that enables the application of an alternating electric field therapy to cancer cells in a 3D extracellular matrix. To demonstrate the potential of our system to aid in designing and testing new therapeutic approaches, cancer cells and cancer cell aggregates were cultured individually or co-cultured with endothelial cells. The metastatic potential of the cancer cells was reduced after electric field treatment. Moreover, the proliferation rate of the treated cancer cells was lower compared with that of the untreated cells, whereas the morphologies and proliferative capacities of the endothelial cells were not significantly affected. These results demonstrate that our novel system can be used to rapidly screen the effect of an alternating electric field on cancer and normal cells within an in vivo-like microenvironment with the potential to optimize treatment protocols and evaluate synergies between tumor-treating field treatment and chemotherapy.
Source Title: Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/srep26584
Rights: Attribution 4.0 International
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