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Title: Early detection of antiangiogenic treatment responses in a mouse xenograft tumor model using quantitative perfusion MRI
Authors: Rajendran, R
Huang, W
Tang, A.M
Liang, J.M
Choo, S
Reese, T
Hentze, H
van Boxtel, S
Cliffe, A
Rogers, K
Henry, B
Chuang, K.H 
Keywords: angiogenesis inhibitor
monoclonal antibody
spin labeling
blood flow
drug screening
Kidney Neoplasms
magnetic resonance angiography
Neovascularization, Pathologic
spin labeling
Angiogenesis Inhibitors
Antibodies, Monoclonal, Humanized
Kidney Neoplasms
Magnetic Resonance Angiography
Neovascularization, Pathologic
Regional Blood Flow
Spin Labels
Xenograft Model Antitumor Assays
Issue Date: 2014
Citation: Rajendran, R, Huang, W, Tang, A.M, Liang, J.M, Choo, S, Reese, T, Hentze, H, van Boxtel, S, Cliffe, A, Rogers, K, Henry, B, Chuang, K.H (2014). Early detection of antiangiogenic treatment responses in a mouse xenograft tumor model using quantitative perfusion MRI. Cancer medicine 3 (1) : 47-60. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Angiogenesis plays a major role in tumor growth and metastasis, with tumor perfusion regarded as a marker for angiogenesis. To evaluate antiangiogenic treatment response in vivo, we investigated arterial spin labeling (ASL) magnetic resonance imaging (MRI) to measure tumor perfusion quantitatively. Chronic and 24-h acute treatment responses to bevacizumab were assessed by ASL and dynamic-contrast-enhanced (DCE) MRI in the A498 xenograft mouse model. After the MRI, tumor vasculature was assessed by CD34 staining. After 39 days of chronic treatment, tumor perfusion decreased to 44.8 ± 16.1 mL/100 g/min (P < 0.05), compared to 92.6 ± 42.9 mL/100 g/min in the control group. In the acute treatment study, tumor perfusion in the treated group decreased from 107.2 ± 32.7 to 73.7 ± 27.8 mL/100 g/min (P < 0.01; two-way analysis of variance), as well as compared with control group post dosing. A significant reduction in vessel density and vessel size was observed after the chronic treatment, while only vessel size was reduced 24 h after acute treatment. The tumor perfusion correlated with vessel size (r = 0.66; P < 0.005) after chronic, but not after acute treatment. The results from DCE-MRI also detected a significant change between treated and control groups in both chronic and acute treatment studies, but not between 0 and 24 h in the acute treatment group. These results indicate that tumor perfusion measured by MRI can detect early vascular responses to antiangiogenic treatment. With its noninvasive and quantitative nature, ASL MRI would be valuable for longitudinal assessment of tumor perfusion and in translation from animal models to human. © 2014 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
Source Title: Cancer medicine
ISSN: 20457634
DOI: 10.1002/cam4.177
Rights: Attribution 4.0 International
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