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Title: Internal motion of chromatin fibers is governed by dynamics of uncompressed linker strands
Authors: Basak, Rajib 
Rosencrans, William
Yadav, Indresh 
Yan, Peiyan
Berezhnoy, Nikolay V
Chen, Qinming
van Kan, Jeroen A 
Nordenskiöld, Lars
Zinchenko, Anatoly
van der Maarel, Johan RC 
Issue Date: 29-Oct-2020
Publisher: Elsevier BV
Citation: Basak, Rajib, Rosencrans, William, Yadav, Indresh, Yan, Peiyan, Berezhnoy, Nikolay V, Chen, Qinming, van Kan, Jeroen A, Nordenskiöld, Lars, Zinchenko, Anatoly, van der Maarel, Johan RC (2020-10-29). Internal motion of chromatin fibers is governed by dynamics of uncompressed linker strands. Biophysical Journal. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Chromatin compaction and internal motion are fundamental aspects of gene expression regulation. Here, we have investigated chromatin fibers comprising recombinant histone octamers reconstituted with double-stranded bacteriophage T4-DNA. The size of the fibers approaches the typical size of genomic topologically associated domains. Atomic force and fluorescence (correlation) microscopy have been used to assess the structural organization, histone-induced compaction, and internal motion. In particular, the fibers are stretched on arrays of nanochannels, each channel with a diameter of 60 or 125 nm. Major intra-fiber segregation and fast internal fluctuations are observed. Full compaction was only achieved by triggering an attractive nucleosome interaction through the addition of magnesium cations. Besides compaction, histone complexation results in a dramatic decrease in the fiber’s relaxation time. The relaxation times are similar to those of naked DNA with a comparable stretch, which indicates that internal motion is governed by the dynamics of uncompressed linker strands. Furthermore, the main reorganization process is association-dissociation of individually compacted regions. We surmise that the modulation of chromatin’s internal motion by histone complexation might have implications for transcriptional bursting.
Source Title: Biophysical Journal
ISSN: 00063495
DOI: 10.1016/j.bpj.2020.10.018
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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