Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2180-10-187
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dc.titleRice bacterial blight pathogen Xanthomonas oryzae pv. oryzae produces multiple DSF-family signals in regulation of virulence factor production
dc.contributor.authorHe, Y.-W
dc.contributor.authorWu, J
dc.contributor.authorCha, J.-S
dc.contributor.authorZhang, L.-H
dc.date.accessioned2020-10-27T11:37:39Z
dc.date.available2020-10-27T11:37:39Z
dc.date.issued2010
dc.identifier.citationHe, Y.-W, Wu, J, Cha, J.-S, Zhang, L.-H (2010). Rice bacterial blight pathogen Xanthomonas oryzae pv. oryzae produces multiple DSF-family signals in regulation of virulence factor production. BMC Microbiology 10 : 187. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2180-10-187
dc.identifier.issn14712180
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/181661
dc.description.abstractBackground. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of rice bacterial blight disease. Xoo produces a range of virulence factors, including EPS, extracellular enzyme, iron-chelating siderophores, and type III-secretion dependent effectors, which are collectively essential for virulence. Genetic and genomics evidence suggest that Xoo might use the diffusible signal factor (DSF) type quorum sensing (QS) system to regulate the virulence factor production. However, little is known about the chemical structure of the DSF-like signal(s) produced by Xoo and the factors influencing the signal production. Results. Xoo genome harbours an rpf cluster comprising rpfB, rpfF, rpfC and rpfG. The proteins encoded by these genes are highly homologous to their counterparts in X. campestris pv. campestris (Xcc), suggesting that Xcc and Xoo might use similar mechanisms for DSF biosynthesis and autoregulation. Consistent with in silico analysis, the rpfF mutant was DSF-deficient and the rpfC mutant produced about 25 times higher DSF-like activity than the wild type Xoo strain KACC10331. From the supernatants of rpfC mutant, we purified three compounds showing strong DSF-like activity. Mass spectrometry and NMR analysis revealed that two of them were the previously characterized DSF and BDSF; the third one was a novel unsaturated fatty acid with 2 double bonds and was designated as CDSF in this study. Further analysis showed that all the three DSF-family signals were synthesized via the enzyme RpfF encoded by Xoo2868. DSF and BDSF at a final concentration of 3 M to the rpfF mutant could fully restore its extracellular xylanase activity and EPS production to the wild type level, but CDSF was less active than DSF and BDSF in induction of EPS and xylanase. DSF and CDSF shared a similar cell density-dependent production time course with the maximum production being detected at 42 h after inoculation, whereas the maximum production of BDSF was observed at 36 h after inoculation. When grown in a rich medium such as YEB, LB, PSA, and NYG, Xoo produced all the three signals with the majority being DSF. Whereas in nutritionally poor XOLN medium Xoo only produced BDSF and DSF but the majority was BDSF. Conclusions. This study demonstrates that Xoo and Xcc share the conserved mechanisms for DSF biosynthesis and autoregulation. Xoo produces DSF, BDSF and CDSF signals in rich media and CDSF is a novel signal in DSF-family with two double bonds. All the three DSF-family signals promote EPS production and xylanase activity in Xoo, but CDSF is less active than its analogues DSF and BDSF. The composition and ratio of the three DSF-family signals produced by Xoo are influenced by the composition of culture media. © 2010 He et al; licensee BioMed Central Ltd.
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceUnpaywall 20201031
dc.subjectB diffusible signal factor
dc.subjectbacterial protein
dc.subjectC diffusible signal factor
dc.subjectcell enzyme
dc.subjectdiffusible signal factor
dc.subjectEPS protein
dc.subjectrpfB protein
dc.subjectrpfC protein
dc.subjectrpfG protein
dc.subjectrppfF protein
dc.subjectunclassified drug
dc.subjectunsaturated fatty acid
dc.subjectvirulence factor
dc.subjectxylan endo 1,3 beta xylosidase
dc.subjectbacterial protein
dc.subjectvirulence factor
dc.subjectarticle
dc.subjectautoregulation
dc.subjectbacterial blight
dc.subjectbacterial strain
dc.subjectbacterial virulence
dc.subjectbiosynthesis
dc.subjectcomputer model
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectenzyme activity
dc.subjectfatty acid synthesis
dc.subjectgene cluster
dc.subjectgenetic transcription
dc.subjectinoculation
dc.subjectmass spectrometry
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance
dc.subjectquorum sensing
dc.subjectrice
dc.subjectsignal transduction
dc.subjectwild type
dc.subjectXanthomonas
dc.subjectXanthomonas campestris
dc.subjectxanthomonas oryzae
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectmultigene family
dc.subjectpathogenicity
dc.subjectplant disease
dc.subjectsignal transduction
dc.subjectBacteria (microorganisms)
dc.subjectXanthomonas oryzae pv. oryzae
dc.subjectBacterial Proteins
dc.subjectGene Expression Regulation, Bacterial
dc.subjectMultigene Family
dc.subjectOryza sativa
dc.subjectPlant Diseases
dc.subjectSignal Transduction
dc.subjectVirulence Factors
dc.subjectXanthomonas
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.1186/1471-2180-10-187
dc.description.sourcetitleBMC Microbiology
dc.description.volume10
dc.description.page187
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